Table 2 Primers sequences used in this study
Nr | Name | Amplification primers (seq) | Sequencing primers | Position in a genome a | Gene (ORF) | Product size range (bp) |
|---|---|---|---|---|---|---|
1 | Clav-VNTR2 | F-5′-GGTCTACGTCGACGAGGTCTT-3′ | F- 5′-GCACCGCCACATGGAGAG-3′ | 3107999-3108175 | putative zinc-dependant oxidoreductase (putative carboxylesterase) | 165-300 |
F-5′-TTCGCGTTCCTCACCAAC-3′ | R-5′-GTCGACGCGCTACGGGAG-3′ | |||||
2 | Clav-VNTR5 | F-5′-GGGCCCGATCAACGACAT-3′ | F-5′-CGGACACGTCAGCCTACC-3′ | 2130562-2130864 | putative transcriptional regulator (MerR family) | 200-475 |
F-5′-CATCGAGTCGGCCCTGGT-3′ | F-5′-GAGATCGCCACGCAGCTC-3′ | |||||
3 | Clav-VNTR9 | F-5′-GCACGGCGTCACGGTCAG-3′ | F-5′-CGAGGAGTGGAACCAGGCCG-3′ | 2183702-2183742 | putative arylesterase (putative transcriptional regulator, LysR-family) | 150-200 |
F-5′-AGCTCGCGAAGCCGTCCAC-3′ | F-5′-CGAAGGCCTCCAAGGGCCAG-3′ | |||||
4 | Clav-VNTR13 | F-5′-GTCGTGGTGCGGGGTCGT-3′ | F-5′-ACGTCCAGCATTCCTCCA-3′ | 468356-468428 | putative NAD(FAD)-dependent dehydrogenase | 200-250 |
F-5′-TGACCGGCACGTCAAGGAGA-3′ | F-5′-ACGTCCAGCATTCCTCCA-3′ | |||||
5 | Clav-VNTR15 | F-5′-GCCGTCTCTGCGTCTTTC-3′ | F-5′-CCTCGAGATGACACCTGAAT-3′ | 2684839-2684928 | putative duplicated acetyltransferase | 130-200 |
F-5′-ATGAGACGTCCAGCAGTGG-3′ | F-5′-GATGTGTACGATCCGCTCTC-3′ | |||||
6 | Clav-VNTR16 | F-5′-GTCGCCTACGAGTTCATGGT-3′ | F-5′-GTCACGGCGCCCTAGGAACC-3′ | 1929615-1929835 | putative glycine/betaine ABC transporter (Putative DNA or RNA helicase) | 175-300 |
F-5′-AGCTCCTCAACAGCCTCGT-3′ | F-5′-TCGGCCAGTGCAGCGTCA-3′ | |||||
7 | Clav-VNTR22 | F-5′-ACACCCGCCCGACTAGACC-3′ | F-5′-GACAGGCCGGTCGGAGGAAT-3′ | 549526-549594 | putative two-component system response regulator | 175-225 |
F-5′-CGGAAGCTGCACGACGAC-3′ | F-5′-GTGCGCGGCGTCGGATAC-3′ | |||||
8 | Clav-VNTR26 | F-5′-CCTTCGCGGTGCGGATCA-3′ | F-5′-GACGAGGACGGTGTCGAG-3′ | 178774-178838 | putative urea amidolyase (conserved hypothetical protein) | 150-175 |
F-5′-GGGATCGTCGACGGCATGAG-3′ | F-5′-GCTGGTGATCGTCTCCAACT-3′ | |||||
9 | gyrB 2 F | F-5′-ACCGTCGAGTTCGACTACGA-3′ | The same as amplification primers | 6588-7113 | DNA gyrase, subunit B | 525 |
gyrB 4R | F-5′- CCTCGGTGTTGCCSARCTT-3′ |  |  |  |  | |
10 | dnaA F | 5-TACGGCTTCGACACCTTCG-3 | The same as amplification primers | 412-1345 | replication initiation factor (RIF) | 933 |
| Â | dnaA R | 5-CGGTGATCTTCTTGTTGGCG-3 | Â | Â | Â | Â |