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Table 2 Primers sequences used in this study

From: Multilocus Variable-Number-Tandem-Repeats Analysis (MLVA) distinguishes a clonal complex of Clavibacter michiganensis subsp. michiganensisstrains isolated from recent outbreaks of bacterial wilt and canker in Belgium

Nr Name Amplification primers (seq) Sequencing primers Position in a genome a Gene (ORF) Product size range (bp)
1 Clav-VNTR2 F-5′-GGTCTACGTCGACGAGGTCTT-3′ F- 5′-GCACCGCCACATGGAGAG-3′ 3107999-3108175 putative zinc-dependant oxidoreductase (putative carboxylesterase) 165-300
F-5′-TTCGCGTTCCTCACCAAC-3′ R-5′-GTCGACGCGCTACGGGAG-3′
2 Clav-VNTR5 F-5′-GGGCCCGATCAACGACAT-3′ F-5′-CGGACACGTCAGCCTACC-3′ 2130562-2130864 putative transcriptional regulator (MerR family) 200-475
F-5′-CATCGAGTCGGCCCTGGT-3′ F-5′-GAGATCGCCACGCAGCTC-3′
3 Clav-VNTR9 F-5′-GCACGGCGTCACGGTCAG-3′ F-5′-CGAGGAGTGGAACCAGGCCG-3′ 2183702-2183742 putative arylesterase (putative transcriptional regulator, LysR-family) 150-200
F-5′-AGCTCGCGAAGCCGTCCAC-3′ F-5′-CGAAGGCCTCCAAGGGCCAG-3′
4 Clav-VNTR13 F-5′-GTCGTGGTGCGGGGTCGT-3′ F-5′-ACGTCCAGCATTCCTCCA-3′ 468356-468428 putative NAD(FAD)-dependent dehydrogenase 200-250
F-5′-TGACCGGCACGTCAAGGAGA-3′ F-5′-ACGTCCAGCATTCCTCCA-3′
5 Clav-VNTR15 F-5′-GCCGTCTCTGCGTCTTTC-3′ F-5′-CCTCGAGATGACACCTGAAT-3′ 2684839-2684928 putative duplicated acetyltransferase 130-200
F-5′-ATGAGACGTCCAGCAGTGG-3′ F-5′-GATGTGTACGATCCGCTCTC-3′
6 Clav-VNTR16 F-5′-GTCGCCTACGAGTTCATGGT-3′ F-5′-GTCACGGCGCCCTAGGAACC-3′ 1929615-1929835 putative glycine/betaine ABC transporter (Putative DNA or RNA helicase) 175-300
F-5′-AGCTCCTCAACAGCCTCGT-3′ F-5′-TCGGCCAGTGCAGCGTCA-3′
7 Clav-VNTR22 F-5′-ACACCCGCCCGACTAGACC-3′ F-5′-GACAGGCCGGTCGGAGGAAT-3′ 549526-549594 putative two-component system response regulator 175-225
F-5′-CGGAAGCTGCACGACGAC-3′ F-5′-GTGCGCGGCGTCGGATAC-3′
8 Clav-VNTR26 F-5′-CCTTCGCGGTGCGGATCA-3′ F-5′-GACGAGGACGGTGTCGAG-3′ 178774-178838 putative urea amidolyase (conserved hypothetical protein) 150-175
F-5′-GGGATCGTCGACGGCATGAG-3′ F-5′-GCTGGTGATCGTCTCCAACT-3′
9 gyrB 2 F F-5′-ACCGTCGAGTTCGACTACGA-3′ The same as amplification primers 6588-7113 DNA gyrase, subunit B 525
gyrB 4R F-5′- CCTCGGTGTTGCCSARCTT-3′     
10 dnaA F 5-TACGGCTTCGACACCTTCG-3 The same as amplification primers 412-1345 replication initiation factor (RIF) 933
  dnaA R 5-CGGTGATCTTCTTGTTGGCG-3     
  1. agenome of Cmm NCPPB 382 (AM711867).