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Table 1 Strains used in this study

From: The mxd operon in Shewanella oneidensis MR-1 is induced in response to starvation and regulated by ArcS/ArcA and BarA/UvrY

Strain

Relevant genotype or description

Source or reference

E. coli

  

S17-lambda pir

thi pro recA hsdR [RP4-2Tc::Mu-Km::tn7]lambda pir Tpr Smr

[38]

AS259 (BW20767)

RP4-2-Tc::Mu-1 Kan::Tn7 integrant leu-63::IS10 recA1 zbf-5 creB510 hsdR17 endA1 thi uidA (deltaMluI)::pir+

[12]

AS262

S17-lambda pir harbouring pUX-BF13

[39]

AS392

S17-lambda pir harbouring pGP704-mini-Tn7(Gm) P A1/04/03-GFPmut3*

[39]

S. oneidensis

  

AS93

S. oneidensis MR-1, wild type, tagged with GFPmut3* in a Tn7 construct, Genr

[12]

AS536

AS93 harbouring pME6031(Tc)::Pmxd-300+1lacZ (pJM1)

This study

AS556

AS93 harbouring pME6031(Tc)::lacZ (promoterless)

This study

AS579 (MR-1)

S. oneidensis MR-1, wild type

PNNL

AS829

In-frame deletion of mxdA in MR-1

This study

AS830

MR-1 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS831

In-frame deletion of mxdB in MR-1 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS832

MR-1 harbouring pME6031(Tc)::Pmxd-300+1lacZ

This study

AS833

MR-1 harbouring pME6031(Tc)::Pmxd-150+1lacZ

This study

AS834

MR-1 harbouring pME6031(Tc)::Pmxd-100+1lacZ

This study

AS835

MR-1 harbouring pME6031(Tc)::Pmxd0+1lacZ

This study

AS837

MR-1 harbouring pProbe-NT(Kan)::Pmxd-300+1gfp

This study

AS838

MR-1 harbouring pProbe-NT(Kan):: gfp (promoterless)

This study

AS839

In-frame deletion of arcA in MR-1

This study

AS840

AS839 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS841

In-frame deletion of arcS in MR-1

This study

AS842

AS841 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS843

In-frame deletion of uvrY in MR-1

This study

AS844

AS843 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS845

In-frame deletion of barA in MR-1

This study

AS846

AS845 tagged with GFPmut3* in a Tn7 construct, Genr

This study

AS855

AS839 harbouring pProbe-NT(Kan)::P mxd -300+1gfp (pJM6)

This study

AS856

AS841 harbouring pProbe-NT(Kan)::P mxd -300+1gfp (pJM6)

This study

AS860

AS841 harbouring pME6031(Tc)::P mxd -300+1lacZ

This study

AS861

AS845 harbouring pME6031(Tc)::P mxd -300+1lacZ

This study

AS862

AS843 harbouring pME6031(Tc)::P mxd -300+1lacZ

This study

AS863

AS839 harbouring pME6031(Tc)::P mxd -300+1lacZ

This study

AS864

AS847 harbouring pME6031(Tc)::P mxd -300+1lacZ

This study

AS865

MR-1 harbouring pME6031(Tc)::lacZ (promoterless)

This study