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Table 4 Oligonucleotides used for the amplification of gene fragments with PCR

From: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

Primer   Sequence (5′-3′) Ta(°C) Protein encoded by the target gene Product size (bp) Reference
pufLF1 CTK TTC GAC TCC TGG GTS GG 56 Reaction center proteins L and M subunits 1500 [5]
pufMR1 CCA TSG TCC AGC GCC AGA A
pufLF2 CTY TTT GAY TTC TGG GTD GG 56 Reaction center proteins L and M subunits 1500 This study
pufMR2 CCA TSG TCC AGC GCC ARA A
PR1 MGN TAY ATH GAY TGG YT 47 Proteorhodopsin opsin subunit 312 [26]
PR2 WWN MGN TAY GTN GAY TGG
PR3 GGR TAD ATN GCC CAN CC
soxB432F-2 GAY GGN GGN GAY MYB TGG 54 Sox enzyme complex B subunit 1000 This study
soxB1446B-2 CAT RTC WCC MCC YTG YTG
rpoB-F AAY CAG TTC CGC GTN GGH YTN GT 52 RNA polymerase beta subunit 1000 This study
rpoB-R AAG TTR TAR CCR TTC CAR GGC AT
  1. The IUPAC nucleotide code is used to indicate wobble positions in degenerate primer sequences. Ta indicates the annealing temperature used in the PCR reaction.