Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

Table 2 Cellular fatty acid patterns of the novel isolate Ivo14 ^T and some related members of the OM60/NOR5 clade

Fatty acid	1	2	3	4	5	6
*Saturated fatty acids*
10:0	―	―	―	―	―	0.9
11:0	0.6	―	1.0	―	0.8	1.6
12:0	5.0	1.0	2.2	1.1	2.3	1.1
13:0	―	0.9	1.0	―	1.2	1.3
14:0	5.4	0.7	2.0	1.8	2.3	2.2
15:0	4.2	7.4	4.9	1.0	4.5	6.6
15:0 ISO	―	―	―	―	0.6	―
16:0	24.0	8.1	5.4	26.8	5.7	11.7
17:0	3.1	5.2	3.1	0.7	5.8	7.0
18:0	―	―	0.6	0.6	―	―
*Unsaturated fatty acids*
15:1 ω6c	―	1.8	2.0	―	4.0	1.1
15:1 ω8c	―	1.3	―	―	0.8	2.7
16:1 ω6c	―	―	6.5	―	―	―
16:1 ω7c	36.1	21.3	23.1	24.4	26.5	18.3
17:1 ω6c	―	5.6	2.8	―	2.3	3.6
17:1 ω8c	―	19.2	8.1	0.7	15.4	15.3
18:1 ω7c	9.7	18.0	29.7	30.0	19.3	19.3
19:1 cyc ω8c	―	―	―	―	0.7	―
*Hydroxy fatty acids*
10:0 3OH	4.8	0.9	2.1	―	2.4	0.8
11:0 3OH	0.6	1.2	―	―	2.5	2.0
12:0 2OH	―	―	―	1.0	―	―
12:0 3OH	2.2	1.1	―	―	1.6	1.3
12:1 3OH	―	1.5	―	2.4	―	―
13:0 3OH	0.7	―	―	―	―	―
Sum in Feature 7	1.3	0.8	2.8	―	―	―

Biomass was obtained by growth of cells on Marine Agar 2216 under fully aerobic conditions. Values are percentages of total fatty acids. Major fatty acids (>5% of total amount) are given in bold. Fatty acids that were detected only in trace amounts (0.5% or less of the total amount) are not shown. The position of the double bond in unsaturated fatty acids is located by counting from the methyl (Ω) end of the carbon chain; cis isomers are indicated by the suffix c; ISO indicates iso-branched fatty acids. Summed feature 7 contained one or more of the following fatty acids that could not be separated by GLC with the MIDI system: 19:1 ω6c, 19:0 cyc and an unknown fatty acid with an equivalent chain length of 18.846. Strains: 1, Luminiphilus syltensis Ivo14^T; 2, Chromatocurvus halotolerans DSM 23344^T; 3, Congregibacter litoralis DSM 17192^T; 4, Pseudohaliea (= Haliea) rubra DSM 19751^T; 5, Haliea salexigens DSM 19537^T; 6, Haliea mediterranea DSM 21924^T.

ISSN: 1471-2180