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Table 1 Distinguishing features of characterized BChl a -containing members of the OM60/NOR5 clade

From: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans

Characteristic 1 2 3 4
Morphology
Size (in SYPHC medium) [μm] 1.2 – 2.2 × 0.6 1.2 – 1.8 × 0.7 1.2 – 1.5 × 0.6 1.2 -1.6 × 0.6
Shape (in SYPHC medium) straight-to-bent rods, coccoid straight-to-bent rods, coccoid straight rods, coccoid straight rods, coccoid
Storage compounds PolyP, PHA PolyP, PHA PolyP, CP PolyP, GLY
Motility - + + -
Cell aggregation - w + +
Pigmentation
BChl a absorption [nm] (in vivo) 801, 871 802, 877 802, 876 804, 821, 871
BChl a production [nmol/mg dw] 1.2 1.1* 3.5 4.4
Carotenoid absorption [nm] (in acetone/methanol) 465, 495, 529 467, 496, 531 465, 495, 529 470, 496, 530
Diffusible brown compound - + - -
Chemotaxonomy
Fatty acid 16:1 ω6c - - + -
Main hydroxy fatty acids (>1% of total fatty acids) 10:0 3OH, 12:0 3OH 11:0 3OH, 12:0 3OH, 12:1 3OH 10:0 3OH 12:1 3OH, 12:0 2OH
Lipoquinones Q8 (tr. Q7) Q8 Q8 Q8*
Polar lipids PG, PE, PN PG, PE, PL, PN PG, PE, PL PG, PE, PL
DNA G + C content [mol%] 57 63* 58 66
Physiology
NaCl range (optimum) [% w/v] 1 – 9 (3) 0 – 18 (4)* 1 – 7 (2) 0.7 – 4.2 (3.5)*
Temp. range (optimum) [°C] 12 – 32 (28) 7 – 40 (37)* 9 – 33 (28) 15 – 44 (30)*
Antibiotic sensitivity
Imipenem (10 μg) + -* + -
Polymyxin B (300 U) + +* + -
Required supplements
L-histidine + - - -
Biotin + +* + +
Thiamin + +* + +
Vitamin B12 + +* + +
Enzyme activities
Catalase + + w +
Oxidase + + [-*] + +
Aesculinase - - - +
Tweenase 20/80 +/w +/w +/w +/+
Urease - - + -
Utilization of
Sucrose - - + -
Glycerol w - w w [-*]
Butanol + - w +
Propionate + + [-*] w + [-*]
Butyrate + + [-*] w +
DL-lactate + - - + [-*]
2-oxoglutarate + - + +
L-serine - - + + [-*]
L-proline - + + -
L-isoleucine - + - +
L-arginine - - + -
L-phenylalanine + - - -
L-glutamate - + + + [-*]
L-glutathione - + + +
  1. All strains were positive in the utilization of acetate, L-alanine, fumarate, DL-3-hydroxybutyrate, DL-malate, oxaloacetate, pyruvate, succinate, and L-threonine. The following compounds were not utilized by all tested strains: citrate, ethanol, formate, D-fructose, D-glucose, glycolate, and methanol. Degradation of starch and gelatin, reduction of nitrate to nitrite and stimulation of growth by thiosulfate were negative in all strains, as well as diagnostic tests for the enzymes tryptophanase and arginine dihydrolase. Data marked with an asterisk were taken from the literature [18, 31]. Published data that disagree with our results are shown in brackets. Abbreviations: PolyP polyphosphate, PHA polyhydroxyalkanoate, CP cyanophycin, GLY glycogen, PG phosphatidylglycerol, PE phosphatidylethanolamine, PL unidentified phospholipid, PN unidentified aminophospholipid, w weakly positive reaction. Strains: 1, Luminiphilus syltensis Ivo14T; 2, Chromatocurvus halotolerans DSM 23344T; 3, Congregibacter litoralis DSM 17192T; 4, Pseudohaliea (= Haliea) rubra DSM 19751T.