Table 1 Oligonucleotides used for the amplification of gene fragments from C. litoralis DSM 17192 T with PCR or semiquantitative RT-PCR
Primer | Sequence (5′-3′) | Ta(°C) | Protein encoded by the target gene | Product size (bp) |
|---|---|---|---|---|
KT71 rpoZ-F | CAT CAC TTC GGC GAG TTC TT | 58 | RNA polymerase omega subunit | 223 |
KT71 rpoZ-R | AGA AGA TTG CCT TGA GTC CG | |||
KT71 cycB1-F | GAC AGT CGG TTT GAT TGC AG | 58 | Cytochrome c5 | 204 |
KT71 cycB1-R | CAT GCG GTG TTG TAA GTT GC | |||
KT71 pufC-F | AAG CAG ACC GAG TGG ACC TA | 58 | Photosynthetic reaction centre cytochrome c subunit | 373 |
KT71 pufC-R | GTG CCT TCT CAG ACT CCG TC | |||
KT71 ctaD-F | ATA TCC ACT TTG GCA CCA GC | 58 | Caa3-type cytochrome c oxidase subunit 1 | 409 |
KT71 ctaD-R | GTG AAG AGC ACA AGG AAG CC | |||
KT71 ccoN1-F | CTT ATC ACC GTC GTC TGG GT | 58 | Cbb3-type cytochrome oxidase CcoN subunit | 392 |
KT71 ccoN-R | GTG TAG TGC AGG TGG TGT GG |