Skip to main content

Table 1 Oligonucleotides used for the amplification of gene fragments from C. litoralis DSM 17192 T with PCR or semiquantitative RT-PCR

From: Mixotrophic growth of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria is carbon-starvation independent and correlates with the type of carbon source and oxygen availability

Primer Sequence (5′-3′) Ta(°C) Protein encoded by the target gene Product size (bp)
KT71 rpoZ-F CAT CAC TTC GGC GAG TTC TT 58 RNA polymerase omega subunit 223
KT71 rpoZ-R AGA AGA TTG CCT TGA GTC CG
KT71 cycB1-F GAC AGT CGG TTT GAT TGC AG 58 Cytochrome c5 204
KT71 cycB1-R CAT GCG GTG TTG TAA GTT GC
KT71 pufC-F AAG CAG ACC GAG TGG ACC TA 58 Photosynthetic reaction centre cytochrome c subunit 373
KT71 pufC-R GTG CCT TCT CAG ACT CCG TC
KT71 ctaD-F ATA TCC ACT TTG GCA CCA GC 58 Caa3-type cytochrome c oxidase subunit 1 409
KT71 ctaD-R GTG AAG AGC ACA AGG AAG CC
KT71 ccoN1-F CTT ATC ACC GTC GTC TGG GT 58 Cbb3-type cytochrome oxidase CcoN subunit 392
KT71 ccoN-R GTG TAG TGC AGG TGG TGT GG
  1. Ta indicates the annealing temperature used in the PCR reaction.
\