Name | Origin and source | Medium used for maintenance and propagation |
---|---|---|
316FNOR 1960 (Vaccine strain) | Obtained from the VLA in 1960 and used in a vaccine trial in goats in Norway during the 1960s [15]. Maintained at the Norwegian Veterinary Institute, Oslo. | Selective Dubos medium [47] supplemented with mycobactin (2 μg/ml) and pyruvate (4 mg/ml) |
316FCYP1966 (Vaccine strain) | Obtained from the VLA in 1966 as lyophilised aliquots and used to vaccinate goats in Cyprus during the 1960s [18]. Strain used in this study was recovered from an aliquot lyophilised on 04 January 1966 and resuscitated in 2009 with limited passage since. | 7H9* |
316FNLD1978 (Vaccine strain) | Obtained from the VLA in 1978 and used as a killed vaccine [38]. Maintained at the Central Veterinary Institute, Lelystad, Netherlands. | Potato starch medium (P.Willemsen personal communication) |
316FNEO4/81 (Vaccine strain) | Neoparasec vaccine (Merial, France) subcultures from a stock [25] assumed to be derived from a 316Â F Weybridge UK strain purchased in the 1980s. | 7H9* or 7H11** |
316FNEO8/81 (Vaccine strain) | ||
316FNEO68451-2 (Vaccine strain) | ||
316FNEO69341 (Vaccine strain) | ||
316v | Australian strain derived from a variant labelled 316f around 1986 [48] which itself was obtained from a New Zealand source who obtained the strain in the early 1980s. Maintained at the University of Sydney, Sydney, Australia. | Mycobactin free medium but grew poorly (R Whittington, personal communication) |
316FUK2000 (Vaccine strain) | Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK, with limited passage. | Liquid Watson Reid†|
316FUK2001 (Vaccine strain) | Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. | 7H11** |
316FNLD2008 (Vaccine strain) | Obtained from VLA in 2008 and maintained at the Central Veterinary Institute, Lelystad, Netherlands | HEYM |
IIUK2000 (Vaccine strain) | Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK. | Liquid Watson Reid†[14] |
IIUK2001 (Vaccine strain) | Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. | 7H11** |
2eUK2000 (Vaccine strain) | Obtained from the VLA in 2000 and maintained at St George’s Hospital Medical School, London, UK. | Liquid Watson Reid ‘A’ Block††medium |
2eUK2001 (Vaccine strain) | Obtained as a lyophilised stock from the VLA in 2001 and maintained at the Moredun Research Institute, Scotland, UK. | 7H11** |
MAPK10 (Wild type strain) | Purchased from ATCC: BAA-968. Sequenced reference strain isolated from a cow in 1990. | 7H9* or 7H11** |
CAM87 (Wild type strain) | MAP Type III strain isolated from a goat in 2005 [26] and maintained at the Universidad Complutense de Madrid, Madrid, Spain. | 7H9* |
JD87/107 (Wild type strain) | Isolated from a deer in 1987 and maintained at the Moredun Research Institute, Scotland, UK. | 7H11** |