Analysis for prevalence and physical linkages amongst integrons, ISEcp1, ISCR1, Tn21 and Tn7 encountered in Escherichia coli strains from hospitalized and non-hospitalized patients in Kenya during a 19-year period (1992–2011)

Table 6 Carriage of transposition genes among Tn 21 transposons

		Number (%) of Tn21transposition gene combination
Category of Tn21	Number of Tn21detected	tnpA + tnpMonly	tnpR + tnpMonly	tnpM + tnpA + tnpR
Tn21 linked to integrons	156	0	9 (6)	147 (94)
Tn21 not linked to integrons	133	56 (42)	63 (47)	14 (11)

PCR methods were used for screening for three genes that are crucial for transposition of Tn21. The tnpA encodes a Tn21-like transposase, the tnpM encodes a putative transposition regulator. Integrons are incorporated into the Tn21 framework adjacent to the tnpM gene. The tnpR encodes a resolvase.

ISSN: 1471-2180