Table 4 Single nucleotide polymorphism (SNP) analysis and dN/dS ratios of categorized and selected coding regions of Pasteurella multocida strains Pm70, P1059, and X73

Pm70 vs. P1059

Total

8910

22111

0.4

Cytoplasmic

2431

9933

0.25

Cytoplasmic membrane

1556

5556

0.28

Extracellular

94

103

0.91

Outer membrane

1575

2062

0.76

Periplasmic

93

549

0.17

Pm70 vs. X73

Total

7401

19304

0.38

Cytoplasmic

2384

9162

0.26

Cytoplasmic membrane

1251

4710

0.27

Extracellular

125

134

0.93

Outer membrane

1783

1976

0.9

Periplasmic

98

593

0.17

Function

Non-synonymous

Synonymous

dN/dS

PfhR (pm0040)

Putative porin-Fe transport

7

15

0.47

PfhB1 (pm0057)

Filamentous hemagglutinin

34

65

0.52

PfhB2 (pm0059)

Filamentous hemagglutinin

498

506

0.98

Est (pm0076)

Outer membrane esterase

39

59

0.66

PtfA (pm0084)

Type IV fimbrial subunit-ptfA

4

0

4

HgbA (pm0300)

TonB-dependent hemoglobin receptor

159

152

1.05

Csy1 (pm0305)

CRISPR-associated protein

290

130

2.23

OmpW (pm0331)

Outer membrane protein

2

4

0.5

pm0336

TonB-dependent receptor

39

57

0.68

HgbB (pm0337)

Hemoglobin binding protein

78

90

0.87

OmpH_1 (pm0388)

Outer membrane porin

36

66

0.55

OmpH_2 (pm0339)

Outer membrane porin

10

16

0.63

TolC1 (pm0527)

Outer membrane efflux channel

12

44

0.27

Pcp (pm0554)

Peptidoglycan-associated protein

0

3

0

HemR (pm0576)

Hemoglobin binding receptor

6

4

1.5

pm0591

Secreted effector protein

75

40

1.88

PhyA (pm0773)

Capular polysacharride export protein

2

4

0.5

OmpA (pm0786)

Outer membrane protein

61

70

0.87

Pm0803

Outer membrane receptor protein, mostly Fe transport

67

58

1.16

TadF (pm0844)

Pilus assembly protein

112

81

1.38

TadE (pm0845)

Pilus assembly protein

134

70

1.91

TadD (pm0846)

Pilus assembly protein

126

103

1.22

RcpB (pm0851)

Pilus assembly protein

144

69

2.08

RcpA (pm0852)

Pilus assembly protein

182

222

0.82

RcpC (pm0853)

Pilus assembly protein

166

112

1.48

Flp1 (pm0855)

Flp pilin component

21

19

1.11

pm0998

Hypothetical protein

6

4

1.5

NanB (pm1000)

Outer membrane sialydase

157

161

0.98

TonB (pm1188)

TonB energy supply via iron transport

3

4

0.75

GlpQ (pm1444)

Glycerophosphodiester

2

3

0.67

PlpE (pm1517)

Protective outer membrane lipoprotein

24

39

0.62

PlpP (pm1518)

Protective outer membrane lipoprotein

63

55

1.13

TorD (pm1794)

Chaperone

4

3

1.33