Table 1 Primers used for cytotoxin-associated gene ( cagA ) and vacuolating cytotoxin gene ( vacA ) typing of H. pylori
Gene target | Primer designation | Nucleotide sequence | Amplicon size (bp) |
|---|---|---|---|
vacAS-F | VacAS-F | 5’-ATGGAAATACAACAAACACAC-3’ | 259 (type s1) |
|  | VacAS-R | 5’-CTGCTTGAATGCGCCAAAC-3’ | 286 (type s2) |
vacA midregion | VacAM-F | 5’-CAATCTGTCCAATCAAGCGAG-3’ | 567 (type m1) |
|  | VacAM-R | 5’-GCGTCAAAATAATTCCAAGG-3’ | 642 (type m2) |
cagA | CagA-F | 5’-GATAACAGGCAAGCTTTTGAGAGGGA-3’ | 393 |
|  | CagA-R | 5’-CCATGAATTTTTGATCCGTTCGG-3’ |  |