Skip to main content

Table 1 Primers used for cytotoxin-associated gene ( cagA ) and vacuolating cytotoxin gene ( vacA ) typing of H. pylori

From: Antibacterial activities of almond skins on cagA-positive and-negative clinical isolates of Helicobacter pylori

Gene target Primer designation Nucleotide sequence Amplicon size (bp)
vacAS-F VacAS-F 5’-ATGGAAATACAACAAACACAC-3’ 259 (type s1)
  VacAS-R 5’-CTGCTTGAATGCGCCAAAC-3’ 286 (type s2)
vacA midregion VacAM-F 5’-CAATCTGTCCAATCAAGCGAG-3’ 567 (type m1)
  VacAM-R 5’-GCGTCAAAATAATTCCAAGG-3’ 642 (type m2)
cagA CagA-F 5’-GATAACAGGCAAGCTTTTGAGAGGGA-3’ 393
  CagA-R 5’-CCATGAATTTTTGATCCGTTCGG-3’