Figure 6
From: Identification of a novel lipin homologue from the parasitic protozoan Trypanosoma brucei
Recombinant TbLpn displays phosphatidic acid phosphatase activity. The enzymatic activity was measured by the release of phosphate from 1,2-dioctanoyl-sn-glycero-3-phosphate (DiC8 PA). The substrate was incubated with increasing amounts of either His-TbLpn (black bars) or His-DEAD (white bars) recombinant proteins. The amount of phosphate released was measured using PiBlue reagent and recording the absorbance at 620 nm.