Figure 3From: Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruziAmastin mRNA expression during the T. cruzi life cycle in different parasite strains. Total RNA was extracted from epimatigote (E), trypomastigote (T) and amastigote forms (A) from CL Brener, Y, G and Sylvio X-10. Electrophoresed RNAs (~10 μg/lane) were transferred to nylon membranes and probed with the 32P- labelled sequences corresponding to δ-amastin, δ-Ama40, β1- and β2-amastins (top panels). Bottom panels show hybridization of the same membranes with a fragment of the 24Sα rRNA.Back to article page