Table 3 Partial 16S rDNA sequences isolated in this study by cloning and PCR-DGGE. The accession number of the closest relative is indicated between brackets.
From: Microbial community of predatory bugs of the genus Macrolophus(Hemiptera: Miridae)
16S rRNA PCR cloning of M. pygmaeus | Â | Â | Â | Â |
|---|---|---|---|---|
Closest known relative | Phylogenetically related class | Sequenced length (bp) | Identity (%) | Accession no. |
Rickettsia limoniae strain Brugge (AF322443) | Alpha-proteobacteria | 1422 | 99 | HE583202 |
Rickettsia bellii (L36103) | Alpha-proteobacteria | 1422 | 99 | HE583203 |
Wolbachia endosymbiont of Culex quinquefasciatus (AM999887) | Alpha-proteobacteria | 1461 | 98 | HE583204 |
Uncultured bacterium (GQ360069) | Gamma-proteobacteria | 1496 | 99 | HE583205 |
Uncultured bacterium (HM812162) | Firmicutes | 767 | 100 | HE583206 |
Uncultured bacterium (FJ512272) | Firmicutes | 764 | 99 | HE583207 |
Uncultured bacterium (GU118480) | Beta-proteobacteria | 743 | 99 | HE583208 |
PCR-DGGE* | Â | Â | Â | Â |
1) Wolbachia endosymbiont of Polydrusus pilifer (JF304463) | Alpha-proteobacteria | 135 | 100 | HE583209 |
2) Rickettsia bellii (L36103) | Alpha-proteobacteria | 135 | 99 | HE583210 |
3) Uncultured bacterium (JF011887) | Gamma-proteobacteria | 160 | 100 | HE583211 |
4) Uncultured bacterium (JF011887) | Gamma-proteobacteria | 160 | 99 | HE583212 |
5) Rickettsia limoniae strain Brugge (AF322443) | Alpha-proteobacteria | 137 | 100 | HE583213 |
6) Uncultured Streptococcus sp. (GU132113) | Firmicutes | 161 | 100 | HE583214 |
7) Uncultured bacterium (FN421660) | Gamma-proteobacteria | 157 | 99 | HE583215 |
8) Rickettsia bellii (L36103) | Alpha-proteobacteria | 135 | 99 | HE583216 |
9) Uncultured bacterium (JF206698) | Gamma-proteobacteria | 160 | 100 | HE583217 |
10) Serratia sp. (HQ891979) | Gamma-proteobacteria | 160 | 100 | HE583218 |
11) Enterobacter cloacae (HQ888762) | Gamma-proteobacteria | 160 | 100 | HE583219 |
12) Serratia sp. (HQ888762) | Gamma-proteobacteria | 160 | 100 | HE583220 |