Table 2 Primer sequences used in this study for PCR and PCR-DGGE. The accession numbers point to the genes that were used to construct the gene specific primers.
From: Microbial community of predatory bugs of the genus Macrolophus(Hemiptera: Miridae)
Targeted gene | Name | Sequence | Accession number/ Reference |
|---|---|---|---|
Cytochrome b gene of Macrolophus spp. | CB-1 | 5’- TATGTACTACCATGAGGACAAATATC -3’ | [68] |
|  | CB-2 | 5’- ATTACACCTCCTAATTTATTAGGAAT -3’ | [68] |
|  | Lau1F | 5’- AATGGCTATGAGGGGGRTTCTC -3’ | [35] |
General primers for the bacterial 16S rRNA gene | 27F | 5’- AGAGTTTGATCMTGGCTCAG -3’ | [43] |
|  | 806R | 5’- GGACTACCAGGGTATCTAAT -3’ | [69] |
|  | 1492R | 5’- TACGGYTACCTTGTTACGACTT -3’ | [43] |
|  | 1525R | 5’- AAAGGAGGTGWTCCARC -3’ | [69] |
V3 region of the bacterial 16S rRNA gene* | 338FGC | 5’- CGCCCGCCGCGCGCGGC | [43] |
| Â | Â | GGGGCGGGGGCACGGGGGG | Â |
|  |  | ACTCCTACGGGAGGCAGCAG -3’ |  |
|  | 518R | 5’- ATTACCGCGGCTGCTGG -3’ | [30] |
wsp gene of Wolbachia | wsp81F | 5’- TGGTCCAATAAGTGATGAAGAAAC -3' | [70] |
|  | wsp691R | 5’- AAAAATTAAACGCTACTCCA -3’ | [70] |
16S rRNA gene of R. limoniae and R. bellii | Rick-1F | 5’- ATACCGAGTGRGTGAYGAAG -3’ | AF322442, L36103 |
16S rRNA gene of R. limoniae | Ricklimoniae-F | 5’- CGGTACCTGACCAAGAAAGC -3’ | AF322442 |
16S rRNA gene of R. bellii | Rickbellii-R | 5’- TCCACGTCGCCGTCTTGC -3’ | L36103 |
Citrate synthase gene (gltA) | gltA133f | 5’- GGTTTTATGTCTACTGCTTCKTG -3’ | [17] |
|  | gltA1197r | 5’- CATTTCTTTCCATTGTGCCATC- 3’ | [17] |
Cytochrome c oxidase gene (coxA) | coxA322f | 5’- GGTGCTCCTGATATGGCATT -3’ | [18] |
|  | coxA1413r | 5’- CATATTCCAACCGGCAAAAG -3’ | [18] |
p-GEMT cloning vector | T7 | 5’- TAATACGACTCACTATAGGG -3’ | Promega |
|  | SP6 | 5’- CTATTTAGGTGACACTATAG -3’ | Promega |