Figure 2

Characteristics of the EST libraries A. Summary of the different EST libraries from Asobara tabida, used to build a transcriptomic map, but also to address the question of the effect of symbiosis and bacterial challenge (b. ch.) on host gene expression. cDNA libraries were sequenced with or without normalization (Norm. or Non norm., respectively). Suppression Subtractive Hybridizations (SSHs) were performed with or without the Mirror Orientation Selection procedure (MOS). The influence of ovarian phenotype was addressed using two different populations known to exhibit extreme phenotypes after Wolbachia removal: females from the Pi3 strain (Pierrefeu, France) do not produce any eggs, while females from the NA strain (Saanich, Canada) produce a few eggs that fail to develop normally. Immune challenge was performed by injecting 1.8x10⁵ Salmonella typhimurium in aposymbiotic females, and RNA was extracted 3h, 6h and 12h after challenge. Abbreviations stand for: DPOv: Distal Part of the Ovaries (e.g. without the eggs), Ov: Ovaries, F: Females, S: Symbiotic, A: Aposymbiotic, C: immune Challenge, NC: No immune Challenge. ESTs: Expressed Sequenced Tags, mito: mitochondrial genes, rRNA: ribosomal RNA, UG: number of unigenes found after a clustering/assembly. B. Abundance classes of ESTs and Unigenes. C. Unigene occurrences among the EST libraries. The horizontal axis represents the different EST libraries. The occurrence of unigenes within the libraries is shown on the vertical axis. A horizontal reading of the graph indicates the percentage of unigenes shared by several EST libraries. D. Gene Ontology (GO) annotation results for High Scoring Pair (HSP) coverage of 0%. GO annotation was first carried out using the Score Function (SF) of the Blast2go software. The GO terms selected by the annotation step were then merged with Interproscan predictions (SF+IPR). Finally, the annex augmentation was run (SF+IPR+ANNEX). E. Annotation distribution of GO terms.