Table 3 Experimental design of S. titanus transmission trials.
No. of individuals (donors + receivers) | Transmission type | Acquisition time | Destination |
|---|---|---|---|
20 (10 + 10) | Co-feeding with Asaia | 24 hours | q-PCR |
38 (19 + 19) | 48 hours | ||
28 (14 + 14) | 72 hours | ||
20 (10 + 10) | 96 hours | ||
8 (4 + 4) | 48 hours | FISH | |
Tot. co-feeders: 114 (57 + 57) | |||
10 (5 + 5) | Asaia Venereal transfer (male to female) | 24 hours | q-PCR |
10 (5 + 5) | 48 hours | ||
10 (5 + 5) | 72 hours | ||
14 (7 + 7) | 96 hours | ||
10 (5 + 5) | 48 hours | FISH | |
10 (5 + 5) | Asaia Venereal transfer (female to male) | 24 hours | q-PCR |
14 (7 + 7) | 48 hours | ||
10 (5 + 5) | 72 hours | ||
12 (6 + 6) | 96 hours | ||
8 (4 + 4) | 48 hours | FISH | |
Tot. mated: 108 (54 + 54) | |||
6 (3 + 3) | Co-housing control trial (males with males) | 24 hours | |
6 (3 + 3) | 48 hours | ||
6 (3 + 3) | 72 hours | ||
6 (3 + 3) | 96 hours | ||
10 (5 + 5) | Co-housing control trial (females with females) | 24 hours | q-PCR |
6 (3 + 3) | 48 hours | ||
6 (3 + 3) | 72 hours | ||
6 (3 + 3) | 96 hours | ||
Tot. co-housed: 52 (26 + 26) | |||
20 (10 + 10) | Negative control for Co-feeding | 24 hours | q-PCR |
22 (11 + 11) | 48 hours | ||
28 (14 + 14) | 72 hours | ||
32 (16 + 16) | 96 hours | ||
10 (5 + 5) | 48 hours | FISH | |
Tot. co-feeders: 112 (56 + 56) | |||
16 (8 + 8) | Negative control for venereal transfer (male to female) | 24 hours | q-PCR |
10 (5 + 5) | 48 hours | ||
8 (4 + 4) | 72 hours | ||
14 (7 + 7) | 96 hours | ||
10 (5 + 5) | 48 hours | FISH | |
8 (4 + 4) | Negative control for venereal transfer (female to male) | 24 hours | q-PCR |
14 (7 + 7) | 48 hours | ||
12 (6 + 6) | 72 hours | ||
10 (5 + 5) | 96 hours | ||
10 (5 + 5) | 48 hours | FISH | |
Tot. mated: 112 (56 + 56) |