Skip to main content

Table 3 Experimental design of S. titanus transmission trials.

From: Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

No. of individuals (donors + receivers) Transmission type Acquisition time Destination
20 (10 + 10) Co-feeding with Asaia 24 hours q-PCR
38 (19 + 19)   48 hours  
28 (14 + 14)   72 hours  
20 (10 + 10)   96 hours  
8 (4 + 4)   48 hours FISH
Tot. co-feeders: 114 (57 + 57)    
10 (5 + 5) Asaia Venereal transfer (male to female) 24 hours q-PCR
10 (5 + 5)   48 hours  
10 (5 + 5)   72 hours  
14 (7 + 7)   96 hours  
10 (5 + 5)   48 hours FISH
10 (5 + 5) Asaia Venereal transfer (female to male) 24 hours q-PCR
14 (7 + 7)   48 hours  
10 (5 + 5)   72 hours  
12 (6 + 6)   96 hours  
8 (4 + 4)   48 hours FISH
Tot. mated: 108 (54 + 54)    
6 (3 + 3) Co-housing control trial (males with males) 24 hours  
6 (3 + 3)   48 hours  
6 (3 + 3)   72 hours  
6 (3 + 3)   96 hours  
10 (5 + 5) Co-housing control trial (females with females) 24 hours q-PCR
6 (3 + 3)   48 hours  
6 (3 + 3)   72 hours  
6 (3 + 3)   96 hours  
Tot. co-housed: 52 (26 + 26)    
20 (10 + 10) Negative control for Co-feeding 24 hours q-PCR
22 (11 + 11)   48 hours  
28 (14 + 14)   72 hours  
32 (16 + 16)   96 hours  
10 (5 + 5)   48 hours FISH
Tot. co-feeders: 112 (56 + 56)    
16 (8 + 8) Negative control for venereal transfer (male to female) 24 hours q-PCR
10 (5 + 5)   48 hours  
8 (4 + 4)   72 hours  
14 (7 + 7)   96 hours  
10 (5 + 5)   48 hours FISH
8 (4 + 4) Negative control for venereal transfer (female to male) 24 hours q-PCR
14 (7 + 7)   48 hours  
12 (6 + 6)   72 hours  
10 (5 + 5)   96 hours  
10 (5 + 5)   48 hours FISH
Tot. mated: 112 (56 + 56)    
  1. Number of insect specimens used for each trial. The duration of the acquisition period, as well as the type of analysis carried out, are indicated both for samples submitted to experiments performed with Gfp-tagged Asaia and for negative controls.