Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

Table 2 Relative abundance of Gfp-tagged Asaia and Asaia sp. within the bacterial community of samples.

	GfpABR					ABR
Sample and transmission type	Average (SD)	24h	48h	72h	96h	Average (SD)	24h	48h	72h	96h
Insect – Donors	0.00724 (0.03573)	-	-	-	-	0.05783	-	-	-	-
Insect –Co-feeding	0.00145 (0.00166)	0.0000004	0.00212	0.00349	0.00019	0.04239 (0.04745)	0.00002	0.08202	0.08490	0.00263
Insect –Venereal transfer, ♂ to ♀	0.00105 (0.00179)	0.0000003	0.00372	0.00004	0.00043	0.02277 (0.02602)	0.05436	0.03381	0.00032	0.00258
Insect –Venereal transfer, ♀ to ♂	0.00137 (0.00025)	-	0.00119	-	0.00155	0.04265 (0.05056)	-	0.07840	-	0.00690
Diet –Co-feeding	0.06143 (0.04979)	0.12291	0.02367	0.08079	0.01833	0.35694 (0.40712)	0.95646	0.09473	0.26633	0.11026
Diet –Venereal transfer, ♂ to ♀	0.00070 (0.00045)			0.00038	0.00102	0.09653 (0.13157)	-	-	0.18957	0.00350
Diet –Venereal transfer, ♀ to ♂	0.00490 (0.00501)	-	0.00135	-	0.00844	0.02983 (0.00491)	-	0.03330	-	0.02636

GfpABR (Gfp-tagged Asaia to Bacteria ratio) calculated as the ratio between the gfp copy number and the 16S rRNA gene copy number of the total bacterial community of the samples. ABR (Asaia to Bacteria ratio) calculated as the ratio between the number of Asaia cells and the total bacteria 16S rRNA gene copy number. In case of insect samples, all of the final copy numbers were calculated per pg of insect 18Sr RNA gene. Values in the Average column represent the average results of each group of trials for insect and diet samples; standard deviation is indicated in parenthesis.

ISSN: 1471-2180