Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

Table 1 Gfp Asaia concentration in S. titanus individuals and in diets.

		insect		diet
		average titre	standard deviation	average titre	standard deviation
	donors	1.1 × 10⁶	2.09 × 10⁶	-	-
Co-feeding	24h	4.75×10 ^-1	8.77 × 10^-1	1.84 × 10²	3.16 × 10²
	48h	2.14 × 10²	5.26 × 10²	3.03 × 10³	5.74 × 10³
	72h	2.67 × 10³	8.01 × 10³	2.22 × 10³	3.25 × 10³
	96h	2.32 × 10⁵	3.28 × 10⁵	3.85 × 10³	6.63 × 10²
	control	0	0	0	0
venereal transfer (male to female)	24h	3.96 × 10^-2	-	0	0
	48h	6.73 × 10^-1	9.48 × 10^-1	0	0
	72h	8.06 × 10⁰	1.32 × 10¹	1.14× 10²	-
	96h	8.96 × 10²	1.79 × 10³	7.27 × 10²	4.57 × 10¹
	control	0	0	0.	0
venereal transfer (female to male)	24h	0	0	0	0
	48h	2.54 ×+02	4.42 × 10²	1.47 10¹	-
	72h	0	0	0	0
	96h	2.53 ×+01	2.41 × 10¹	4.13 × 10²	5.61 × 10²
	control	0	0	0	0
Co-housing	24h	0	0	0	0
	48h	0	0	0	0
	72h	0	0	0	0
	96h	0	0	0	0

The concentration of Gfp Asaia in insect and diet samples as indicated by the number of gfp gene copies per positive sample. In case of insect samples, the gfp copy number was calculated per pg of insect 18Sr RNA gene, while for diets it was calculated per ng of total DNA. Average values and standard deviation are shown for each trial. Standard deviation is missing when the number of positive samples was <2.

ISSN: 1471-2180