Relative abundance of Gfp-Asaia within the whole Asaia populations. The relative abundance of the tagged strain in total Asaia community is calculated by the ratio between the number of gfp gene copies per sample and the number of Asaia cells (which is Asaia 16S rRNA gene copies divided by four, assuming that four rRNA gene copies per cell are present in Asaia, as reported in Crotti et al. ) per sample. In each graph white columns represent S. titanus individuals, and grey columns represent diets. The “donors” columns refer to average values of donor insects in all trials. “24h”, “48h”, “72h”, and “96h” indicate the time of exposure to co-feeding or the time of incubation after mating with infected individuals. The Gfp-tagged Asaia to total Asaia ratio is indicated in insects and diets submitted to co-feeding trials (A), and to venereal transmission experiments, from male to female (B) and from female to male (C), respectively. The bars on each column represent the standard error.