Figure 1

Gfp-Asaia infection rates and density within infected samples. White columns represent S. titanus individuals, and grey columns represent diets. The “donors” columns refer to the average values of donor insects in all of the trials. “24h”, “48h”, “72h”, and “96h” indicate the time of exposure to co-feeding or the time of incubation after mating with infected individuals. The “control” columns represent the values obtained from insects fed on sterile sugar diets, as well as those obtained from individuals co-housed with Gfp Asaia-infected specimens of the same sex. A-C) Percentage of insects and diets colonized by Gfp-tagged Asaia. D-F) Transformed (10 + log) number of gfp gene copies per positive sample. Bars represent the standard error of transformed data. Different letters (black for insect and grey for diet samples) indicate significantly different values (ANOVA, P<0.05).