Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina)

Table 2 Wolbachia MLST allelic profiles for 11 populations of Glossina

Code	Species	Country (area, collection date)	Wolbachia MLST
			ST	gatB	coxA	hcpA	ftsZ	fbpA
12.3A	G. m. morsitans	Zambia (MFWE, Eastern Zambia, 2007)	226	141	127	23	114	15
32.3D	G. m. morsitans	Zimbabwe (Makuti, 2006)	226	141	127	23	114	15
GmcY	G. m. centralis	Yale lab-colony (2008)	226	141	127	23	114	15
30.9D	G. m. morsitans	Zimbabwe (Rukomeshi, 2006)	227	141	127	23	115	15
GmmY	G. m. morsitans	Yale lab-colony (2008)	228	8	127	23	113	15
24.4A	G. m. morsitans	KARI-TRC lab-colony (2008)	229	142	128	23	113	15
09.7G	G. brevipalpis	Seibersdorf lab-colony (1995)	230	143	129	23	56	15
05.2B	G. austeni	South Africa (Zululand, 1999)	231	128	109	127	98	20
GauK	G. austeni	Kenya (Shimba Hills, 2010)	197	128	108	127	98	20
15.5B	G. pallidipes	Ethiopia (Arba Minch, 2007)	232	144	47	149	116	202
405.11F	G. p. gambiensis	Guinea (Kindoya, 2009)	233	145	130	150	117	203

Identical nucleotide sequences at a given locus for different strain were assigned the same arbitrary allele number. Each strain was then identified by the combination of the five MLST allelic numbers, representing its allelic profile. Each unique allelic profile was assigned an ST (Sequence Type), which ultimately characterizes a strain [41].

ISSN: 1471-2180