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Figure 3 | BMC Microbiology

Figure 3

From: Delayed larval development in Anopheles mosquitoes deprived of Asaiabacterial symbionts

Figure 3

Normal developmental time of mosquitoes is associated with amplification bands from Asaia in PCR-DGGE. PCR-DGGE carried out on pupae and freshly molted adults of An. stephensi from the experimental groups of this study. a: DGGE on the non treated larvae. b: DGGE on the larvae treated with rifampicin at 120 μg ml-1. c: DGGE on larvae treated with rifampicin at 120 μg ml-1 and supplemented with rifampicin-resistant Asaia. I: bands at this level were identified as Asaia sp. after sequencing. II: bands at this level were identified as Burkholderia sp. III: bands at this level were identified as Delftia sp. Sequencing of bands at level VI was unsuccessful. V bands at this level were identified as Anopheles sp. 18S. * indicate the position in the gel form the larvae treated with antibiotics where Asaia bands were expected.

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