Visualisation of acridine orange (AO)- and TUNEL-stained germarium cells of D. melanogaster . A, C, E, G, germaria containing apoptotic cells in region 2a/2b from 5 day-old uninfected (A, E) and Wolbachia-infected (C, G) females (AO staining). B, D, F, H, germaria not containing apoptotic cells from the same fly stocks (AO staining). Arrows indicate small punctate AO-staining in regions 1 and 2a/2b (C, D, G, H). I, relative proportion of germaria containing apoptotic cells from ovaries of the uninfected (w1118T, Canton ST) and Wolbachia-infected (w1118, Canton S) flies. The total number of examined germaria is indicated by blue number; bars show the average percentage per experiment ± s. e. m. J, L, germaria containing apoptotic cells in region 2a/2b in the wMelPop- and wMel-infected fly stocks, respectively (TUNEL). K, M, germaria not containing apoptotic cells from the same fly stocks. Region 2a/2b of the germarium is indicated by red brackets. Scale bars: 20 μm.