Analysis of gene expression profiles in the bacteriome. Transcripts of genes were quantified by qRT-PCR. Bacteriomes dissected from fourth-instar larvae were compared to whole aposymbiotic fourth-instar larvae. Expression of genes was normalized with the expression of the ribosomal protein L29. Each box represents the median (bolt line) and the quartiles (25% / 75%) of five independent measurements. Statistical analysis was performed with the REST pair-wise fixed reallocation randomization test. Asterisks indicate a significant difference between the bacteriome and the control (p-value < 0.05).