Wolbachia surface protein induces innate immune responses in mosquito cells

BMC Microbiology

Table 1 Primers used in qRT-PCR

	Forward primer	Reverse primer
*An gambiae*
APL1	ACCAGCCGCAGTTTGATAG	CAATCCCAGTCATTATGCGA
RpS7^, CEC1, DEF1 ref* [21]and GAMB, TEP1, FBN9 ref [22]
*Ae albopictus* ^!
DEF (D) ^*	TTCGATGAACTACCGGAGGA	AGCACAAGCACTGTCACCAA
RpL17^*	AGTGCGTTCCATTCCGTC	CTTCAGCGTTCTTCAACAGC
CEC (A1), TEP (20), PGRP (SP1) and CLIP (B37) ref [23]

*RpS7 was used as the reference gene in An. gambiae analysis while RpL17 was the reference for Ae. albopictus. ^!The Ae albopictus immune gene primers have been determined via degeneracy against the corresponding Ae. aegyptiorthologous genes shown in brackets.

ISSN: 1471-2180