Figure 5
From: Anti-staphylococcal activities of lysostaphin and LytM catalytic domain
Effect of buffer pH on lytic activity of lysostaphin and LytM 185-316. Activity of lysostaphin (solid lines) and LytM185-316 (dotted lines) in 50 mM Tris buffer at pH 7.0 (squares), 8.0 (circles) and 9.0 (triangles). S. aureus cells were collected in the exponential growth phase, washed and resuspended in test buffer to apparent OD595 ~1.8. The addition of LytM185-316 or lysostaphin (both at 18 nM final concentration) led to cell lysis, which reduced light scattering and thus apparent OD595. As some decrease was also observed in the absence of enzyme, all OD595 values were expressed as percent of the control without enzyme.