Figure 2
From: Anti-staphylococcal activities of lysostaphin and LytM catalytic domain
Kinetics of S. aureus infection in mouse model and the effect of enzyme treatment. Colony forming units (CFUs) after S. aureus infection. The data are represented in whisker-box plots. Boxes cover the second and third quartiles, and horizontal lines indicate medians. (A) Persistence of S. aureus strain LS-1 in eczematous ears of NMRI mice 1, 2, 3, and 6 days after topical application of 106 S. aureus LS-1 per ear (n = 4/time point). (B) Effect of lysostaphin (Lss) and LytM185-316 (LytM) on S. aureus P1 recovery from infected mice ears as compared to the control. Twelve hours after inoculation of bacteria on ears with eczema 100 μg of lysostaphin or LytM185-316 (100ug each) in 50 mM glycine pH 8.0 and 10% glycerol buffer was applied to each mice ear. Ears of control mice were treated with buffer alone. Treatment was repeated 4 times every 12 hours and ears were examined 3 hours after the last treatment. The two-tailed Student's t-test (assuming equal variances in all samples) was used to calculate probabilities for the null hypothesis of equal means in pairwise comparisons. The resulting p-values are indicated above the curly brackets.