Table 1 Comparisons of YMDD variants in serum of patients with acute and chronic HBV infection detected by direct sequencing and pyrosequencing
Patient number | Type of infection | Treatment | Duration (months) | Viral load (cp/mL) | Sub genotype | Direct sequencing | Pyrosequencing % amino acid | |||
|---|---|---|---|---|---|---|---|---|---|---|
WT | MUT | M | V | I | ||||||
ATG | Codon | ATG | (codon) | (codon)/(codon) | ||||||
1969 | acute | - | - | 1.1x106 | A1 | M/ATG | - | 100 | - | - |
2098 | acute | - | - | 1.4x106 | A1 | M/ATG | - | 100 | - | - |
1377 | acute | - | - | 3.5x104 | A1 | M/ATG | - | 100 | - | - |
1504 | acute | - | - | 6.2x102 | A1 | M/ATG | - | 100 | - | - |
1379 | acute | - | - | 2.8x104 | A1 | M/ATG | - | 95 | - | 5 (ATT) |
1419 | acute | - | - | 6.5x103 | A1 | M/ATG | - | 100 | - | - |
1781 | acute | - | - | 6.6x102 | A1 | M/ATG | - | 95 | - | 5 (ATT) |
1510 | acute | - | - | 8.6x103 | A1 | M/ATG | - | 83 | - | 17 (ATA) |
1384 | acute | - | - | 3.3x105 | A2 | M/ATG | - | 100 | - | - |
2190 | acute | - | - | - | A2 | M/ATG | - | 94 | 6 (GTT) | - |
603 | acute | - | - | 1.2x105 | A2 | M/ATG | - | 100 | - | - |
1472 | acute | - | - | 3.0x103 | A2 | M/ATG | - | 100 | - | - |
1386 | acute | - | - | 1.3x105 | A2 | M/ATG | - | 96 | - | 4 (ATT) |
1120 | acute | - | - | 7.1x102 | A2 | M/ATG | - | 93 | - | 7 (ATT) |
1393 | acute | - | - | 7.2x103 | A2 | M/ATG | - | 100 | - | - |
1889 | acute | - | - | 5.6x105 | A2 | M/ATG | - | 96 | - | 4 (ATT) |
1474 | acute | - | - | 5x104 | D2 | M/ATG | - | 100 | - | - |
1980 | acute | - | - | 2.5x103 | D2 | M/ATG | - | 94 | 6 (GTT) | - |
1314 | acute | - | - | 2.0x104 | D3 | M/ATG | - | 100 | - | - |
1570 | acute | - | - | 1.3x103 | F2 | M/ATG | - | 94 | - | 6 (ATT) |
NN003 | chronic | LAM | 01 | 3.7x104 | A1 | M/ATG | 94 | - | 6 (ATT) | |
NN004 | chronic | LAM | 06 | 1.7 x104 | A1 | M/ATG | 96 | - | 4 (ATT) | |
NN124 | chronic | LAM | 06 | 9.7 x102 | A1 | - | V/GTG | - | 40 (GTG) | 60 (ATT) |
NN092 | chronic | LAM | 07 | 7.6 x106 | A1 | M/ATG | - | 100 | - | - |
NN006 | chronic | LAM + TDF | 12 | 1.7 x104 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN026 | chronic | LAM | 12 | 1.2 x107 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN041 | chronic | LAM | 12 | 1.3 x104 | A1 | M/ATG | - | 94 | - | 6 (ATT) |
NN043 | chronic | LAM | 12 | 4.2 x105 | A1 | M/ATG | - | 100 | - | - |
NN132 | chronic | LAM | 12 | 9.4 x102 | A1 | - | V/GTG | 10 | 75 (GTG) | 15 (ATT) |
NN123 | chronic | LAM | 18 | 2.4 x109 | A1 | M/ATG | - | 94 | - | 6 (ATA) |
NN009 | chronic | LAM | 24 | 2.1 x104 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN024 | chronic | LAM | 24 | 5.2 x107 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN010 | chronic | LAM | 36 | 4.9 x105 | A1 | - | I/ATT | 3 | - | 93 (ATT)/4 (ATA) |
NN018 | chronic | LAM | 36 | 4.6 x103 | A1 | - | V/GTG | 6 | 94 (GTG) | - |
NN019 | chronic | LAM | 36 | 3.0 x103 | A1 | M/ATG | - | 96 | - | 4 (ATA) |
NN027 | chronic | LAM | 36 | 2.8 x104 | A1 | M/ATG | - | 95 | - | 5 (ATT) |
NN037 | chronic | LAM | 36 | 4.8 x105 | A1 | M/ATG | - | 100 | - | - |
NN079 | chronic | LAM | 36 | 9.6 x103 | A1 | M/ATG | - | 100 | - | - |
NN087 | chronic | LAM | 72 | 1.1 x104 | A1 | M/ATG | - | 100 | - | - |
NN007 | chronic | LAM | 84 | 2.8 x104 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN028 | chronic | LAM | 108 | 1.8 x109 | A1 | V/GTG | - | 100 (GTG) | - | |
NN032 | chronic | LAM + TDF | 132 | 1.2 x104 | A1 | - | V/GTG | - | 100 (GTG) | - |
NN025 | chronic | LAM | 05 | 4.3 x104 | A2 | M/ATG | - | 100 | - | - |
NN014 | chronic | LAM | 07 | 1.4 x105 | A2 | - | I/ATT | - | - | 100 (ATT) |
NN042 | chronic | LAM | 12 | 5.4 x107 | A2 | - | V/GTG | 6 | 94 (GTG) | - |
NN022 | chronic | LAM + ADV | 24 | 1.7 x105 | B | - | I/ATT | - | 25 (GTG) | 70 (ATT) |
NN074 | chronic | LAM | 06 | 6.5 x105 | D2 | - | V/GTG | - | 100 (GTG) | - |
NN125 | chronic | LAM + TDF | 12 | 2.5 x103 | D2 | - | I/ATT | - | - | 100 (ATT) |
NN001 | chronic | LAM | 60 | 2.4x104 | D3 | - | V/GTG | - | 100 (GTG) | - |
NN091 | chronic | LAM | 06 | 4.3 x103 | D3 | - | I/ATT | - | - | 100 (ATT) |
NN096 | chronic | LAM | 06 | 3.1 x103 | D3 | M/ATG | - | 100 | - | - |
NN097 | chronic | LAM | 06 | 5.3 x106 | D3 | M/ATG | - | 95 | - | 5 (ATT) |
NN129 | chronic | LAM | 06 | 7.2 x108 | D3 | - | V/GTG | - | 95 (GTG) | 5 (ATT) |
NN029 | chronic | LAM | 12 | 7.0 x104 | D3 | M/ATG | - | 86 | 4 (GTG) | 6 (ATA)/4 (ATT) |
NN038 | chronic | LAM + TDF | 12 | 2.9 x105 | D3 | M/ATG | - | 100 | - | - |
NN077 | chronic | LAM | 12 | 9.7 x105 | D3 | - | I/ATT | 4 | - | 96 (ATT) |
NN034 | chronic | LAM + ADV | 24 | 1.0 x105 | D3 | - | V/GTG | - | 90 (GTG) | 10 (ATT) |
NN075 | chronic | LAM | 60 | 3.2 x103 | D3 | M/ATG | - | 100 | - | - |
NN031 | chronic | LAM | 72 | 6.8 x107 | D3 | - | V/GTG | - | 100 (GTG) | - |
NN126 | chronic | LAM | 06 | 1.9 x108 | F1b | - | I/ATC | - | 30 (GTG) | 70 (ATC) |
NN105 | chronic | LAM | 06 | 3.7 x108 | F2 | - | V/GTG | - | 100 (GTG) | - |
NN078 | chronic | LAM | 12 | 1.2 x106 | F2 | M/ATG | - | 95 | - | 5 (ATT) |
NN134 | chronic | LAM | 12 | 2.7 x104 | F2 | - | I/ATT | - | 25 (GTG) | 75 (ATT) |
NN020 | chronic | LAM | 48 | 3.7 x104 | F2 | M/ATG | - | 100 | - | - |