Figure 2

RDA-visualization of PCR-DGGE profile similarities. RDA visualization of microbiota profile similarities and ABO blood group types, revealing a clustering of the samples. Each dot represents a single individual and diamonds mark the calculated data centre points of the corresponding blood groups. P-value marks the statistical significance of the difference between blood group centres, computed with ANOVA-like permutation test from PCR-DGGE intensities grouped by ABO blood group (A) or by the presence of B-antigen (B). Dot colours for the ABO blood groups are as follows: A = red, B = blue, AB = green and O = black and for the B-antigen = blue and non-B antigen red, respectively. UNIV represent the PCR-DGGE obtained with the universal eubacterial primers (dominant bacteria), EREC with the Eubacterium rectale – Clostridium coccoides primers and CLEPT with the Clostridium leptum primers. The RDA analysis shows clustering of samples according to ABO blood groups, especially according to the presence of the B antigen in the dominant and EREC group microbiota.