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Table 2 Primers and probes tested in this study

From: Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil

Primer/Probe

Sequence (5′-3′)

Length (bp)

Amplicon (bp)

Target region

GC (%)

TmgITS1for

GCGTCTCCGAATCCTGAATA

20

106

ITS1

50

TmgITS1rev

ACAGTAGTTTTTGGGACTGTGC

22

  

45

TmgITS1prob

TGTACCATGCCATGTTGCTT

20

  

45

TmgITS2for

AAACCCACTCACGGAATCAC

20

99

ITS2

50

TmgITS2rev

CGTCATCCTCCCAATGAAA

19

  

47

TmgITS2prob

GTACCAAGCCACCTCCATCA

20

  

55