Figure 1
Sensitivity and reproducibility of the method of characterization of Coxiella burnetii. Reproducibility. Lanes 1–6: Isolates NMI, CS-27, local cattle isolate 273, Priscilla, SQ217 and F2, passage “n”; lanes 7–12: same isolates, passage “n+10”. Results using clinical, veterinary and arthropod samples. Lane 13: specimen of R. sanguineus (M28CE4GA7C); lanes 14–16: specimens of H. lusitanicum (M28P1GA8A, M28PE14GV5C and M28PE14GV5F); lane 17: specimen of D. marginatus (M28P2GA45C); lane 18: human serum (2172); lane 19: sheep placenta (70924); lane 20: goat lung (67025); lane 21: cattle endocervical exudate (70814); lane 22: human clot (BZO18); lane 23: human plasma (0904); lane 24: rat liver (78); lane 25: negative control. Sensitivity. Lanes 26–28: 103, 102 and 10 genome equivalents of isolate NMII. Left panel: position of the probes for each ORF.