Primers | Sequence | Restriction site | target |
---|---|---|---|
General PCR amplification | |||
310 F | CGTACAGTCGACTTAGTTCTGGCAATCAGGACA | SalI | perR |
310R | CGTATCGGATCCATGGAACTCCATTCTCACTTC | BamHI | Â |
310 L01: | TAGTAAGCTTCACAGTTGGACCTTGGTT | Hind III | Left arm of perR |
310 L02 | TCACCTGCAGCGGCATTTGTCCTGATTG | PstI |  |
310R01 | TCACCTGCAGTTAGCATTGAAGTGAGAATGG | PstI | Right arm of perR |
310R02 | AGGTGAATTCTTGCTACTGTAATGGTCG | EcoRI | Â |
ermF | TCACCTGCAGGAGTGTGTTGATAGTGCA | PstI | erm r |
ermR | AGGTCTGCAGCTTGGAAGCTGTCAGTAG | PstI | Â |
C310F | TCACCTGCAGGATGATGTGGCTGTGTTG | PstI | perR and its promoter |
C310R | TAGTGGATCCAAGTCATGTCCGTCGTAG | BamHI | Â |
PdprF | TCAGAATTCTCGGGCTATAGGTAAAAG | EcoRI | Promoter of dpr |
PdprR | TCAGGATCCATATCACCCTTTCTTTTATT | BamHI | Â |
EGFP 01 | TCAGGATCCATGAGTAAAGGAGAAGAAC | BamHI | EGFP gene |
EGFP 02 | TCACTGCAGTGCTATTTGTATAGTTCATC | PstI | Â |
1772P01 | TCCAGGACTGGTGGCGAC | Â | Promoter of 1772 |
1772P02 | AAAATGATCTCCTTAAATTA | Â | Â |
relAP01 | CATATCTCTACTCTTCCTC | Â | Promoter of relA |
relAP02 | AGCTAGTGTGAGTGCTAC | Â | Â |
gidAP01 | CATGTTGTTCTCTCCTTC | Â | Promoter of gidA |
gidAP02 | TTGAGGTCAATGAGGTAG | Â | Â |
Real-time RT-PCR | Â | Â | |
0309 F | GCAACACTTTCTGCCATCA |  | pmtA |
0309R | GGTCGCACCTACAACTTCA | Â | Â |
1771 F | CGCACCAATCCGTCTTTA |  | metQ |
1771R | TTTCGTTTGTTGGGTCGT | Â | Â |
2094 F | TAAGACCGACGAATCCC |  | relA |
2094R | TCATCCGCGACAGCT | Â | Â |
1689 F | TTTATCAGTAGCCCATTCA |  | dpr |
1689R | AAACGCTCACTCATCTCA | Â | Â |
1539 F | AGAAGGCAAGTTGGAAG |  | sodA |
1539R | GTAGTTTGGACGGACATT | Â | Â |
0155 F | AGAAGTAAACGCTGCTAT |  | gapdh |
0155R | CAAACAATGAACCGAAT | Â | Â |