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Table 4 Primers used in this study

From: A Fur-like protein PerR regulates two oxidative stress response related operons dpr and metQIN in Streptococcus suis

Primers

Sequence

Restriction site

target

General PCR amplification

310 F

CGTACAGTCGACTTAGTTCTGGCAATCAGGACA

SalI

perR

310R

CGTATCGGATCCATGGAACTCCATTCTCACTTC

BamHI

 

310 L01:

TAGTAAGCTTCACAGTTGGACCTTGGTT

Hind III

Left arm of perR

310 L02

TCACCTGCAGCGGCATTTGTCCTGATTG

PstI

 

310R01

TCACCTGCAGTTAGCATTGAAGTGAGAATGG

PstI

Right arm of perR

310R02

AGGTGAATTCTTGCTACTGTAATGGTCG

EcoRI

 

ermF

TCACCTGCAGGAGTGTGTTGATAGTGCA

PstI

erm r

ermR

AGGTCTGCAGCTTGGAAGCTGTCAGTAG

PstI

 

C310F

TCACCTGCAGGATGATGTGGCTGTGTTG

PstI

perR and its promoter

C310R

TAGTGGATCCAAGTCATGTCCGTCGTAG

BamHI

 

PdprF

TCAGAATTCTCGGGCTATAGGTAAAAG

EcoRI

Promoter of dpr

PdprR

TCAGGATCCATATCACCCTTTCTTTTATT

BamHI

 

EGFP 01

TCAGGATCCATGAGTAAAGGAGAAGAAC

BamHI

EGFP gene

EGFP 02

TCACTGCAGTGCTATTTGTATAGTTCATC

PstI

 

1772P01

TCCAGGACTGGTGGCGAC

 

Promoter of 1772

1772P02

AAAATGATCTCCTTAAATTA

  

relAP01

CATATCTCTACTCTTCCTC

 

Promoter of relA

relAP02

AGCTAGTGTGAGTGCTAC

  

gidAP01

CATGTTGTTCTCTCCTTC

 

Promoter of gidA

gidAP02

TTGAGGTCAATGAGGTAG

  

Real-time RT-PCR

  

0309 F

GCAACACTTTCTGCCATCA

 

pmtA

0309R

GGTCGCACCTACAACTTCA

  

1771 F

CGCACCAATCCGTCTTTA

 

metQ

1771R

TTTCGTTTGTTGGGTCGT

  

2094 F

TAAGACCGACGAATCCC

 

relA

2094R

TCATCCGCGACAGCT

  

1689 F

TTTATCAGTAGCCCATTCA

 

dpr

1689R

AAACGCTCACTCATCTCA

  

1539 F

AGAAGGCAAGTTGGAAG

 

sodA

1539R

GTAGTTTGGACGGACATT

  

0155 F

AGAAGTAAACGCTGCTAT

 

gapdh

0155R

CAAACAATGAACCGAAT

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