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Table 1 The results of PerR regulon’s identification

From: A Fur-like protein PerR regulates two oxidative stress response related operons dpr and metQIN in Streptococcus suis

Predicted target genesa

Gene names

Function of genes

Predicted PerR-box

NTANAANNATTNTAN

qRT-PCRb

EMSA results

SSU05_0022

 

aromatic amino acid aminotransferase

ATAAAACTATTATAA

−2.5 (0.6)

 

SSU05_0209

 

hypothetical protein

CTATAATCATTTTAT

+1.1 (0.2)

 

SSU05_0308

 

hypothetical protein

GTAAAATTATTATAA

−1.1 (0.1)

 

SSU05_0309

pmtA

cation transport ATPase

TTAGAATTATTATAA

TTATAACGATTATAA

−1.1 (0.1)

negative

SSU05_0618

 

MATE efflux family protein

TTAAAATAATTATAA

−4.2 (1.1)

 

SSU05_1264

 

SAM-dependent methyltransferase

ATAGAATTATTATAA

−1.1 (0.3)

 

SSU05_1265

 

sulfatase

ATAGAATTATTATAA

−1.8 (0.3)

 

SSU05_1341

lacI

LacI family transcriptional regulator

TTAGAATCATTCTAG

−1.8 (0.4)

 

SSU05_1689

dpr

peroxide resistance protein

TTATAATTATTATAA

+9.3 (1.1)

positive

SSU05_1691

 

phosphotyrosine protein phosphatase

TTATAATTATTATAA

−1.7 (0.4)

 

SSU05_1771

metQ

lipoprotein transporter

ATACAATGATTGTAA

+4.0 (0.2)

positive

SSU05_1855

escA

ABC transporter ATP-binding protein

ATATAATTATTATAA

−16.1 (5.2)

 

SSU05_1856

 

HIT-family protein

ATATAATTATTATAA

−1.6 (0.4)

 

SSU05_2094

relA

GTP pyrophosphokinase

GTATAATGATTGTAG

+2.1 (0.6)

negative

SSU05_2095

cpdB

2',3'-cyclic-nucleotide 2'-phosphodiesterase

GTATAATGATTGTAG

−3.0 (1.1)

 

SSU05_2112

 

hypothetical protein

GTATAATGATTATAC

−1.5 (0.6)

 

SSU05_2113

rarA

recombination factor protein

GTATAATGATTATAC

+1.7 (0.5)

 

SSU05_2191

rlmH

rRNA large subunit methyltransferase

ATAAAATAATTGTAA

−1.3 (0.3)

 

SSU05_2192

htrA

trypsin-like serine protease

ATAAAATAATTGTAA

+1.2 (0.3)

 
  1. aS. suis ORF number of S. suis 05ZYH33
  2. bFold-change (standard deviation) of expression in ΔperR compared to expression in wild-type