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Table 1 Primers for Quantitative PCR

From: Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests

PCR Reference Primers Target gene Cycling conditions Concentration
L. species Zariffard MR [28] F-LBF: 5′- ATGGAAGAACACCAGTGGCG-3′ 16 S r RNA 15 min 95 °C, (15 sec 95 °C, 45 sec 50 °C, 45 sec 72 °C) x37 150 nM
R- LBR: 5′- CAGCACTGAGAGGCGGAAAC-3′
L. crispatus Byun R [29] LcrisF: 5′-AGCGAGCGGAACTAACAGATTTAC-3′ 16 S r RNA 15 min, 95 °C, (15 sec 95 °C, 60 sec 60 °C, 20 sec 72 °C) x40 100 nM
LcrisR : 5′-AGCTGATCATGCGATCTGCTT-3′
L. gasseri Tamrakar R [30] LgassF: 5′-AGCGAGCTTGCCTAGATGAATTTG-3′ 16 S r RNA 15 min 95 °C, (15 sec 95 °C, 60 sec 57 °C, 60 sec 65 °C) x40 200 nM
LgassR: 5′-TCTTTTAAACTCTAGACATGCGTC-3′
L. iners De Backer E [31] InersFw: 5′-GTCTGCCTTGAAGATCGG-3′ 16 S r RNA 15 min 95 °C, (15 sec 95 °C, 55 sec 60 °C, 60 sec 65 °C) x35 200 nM
InersRev: 5′-ACAGTTGATAGGCATCATC-3′
L. jensenii Tamrakar R [30] LjensF: 5′-AAGTCGAGCGAGCTTGCCTATAGA-3′ 16 S r RNA 15 min 95 °C, (15 sec 95 °C, 55 sec 60 °C, 60 sec 72 °C) x40 300 nM
LjensR: 5′-CTTCTTTCATGCGAAAGTAGC-3′
L. vaginalis In-house designed primers LV16s_23s_F: 5′-GCCTAACCATTTGGAGGG-3′ 16 S-23 S r RNA 15 min 95 °C, (15 sec 95 °C, 30 sec 56 °C, 30 sec 72 °C)x37 200 nM
LV16s_23s_R3: 5′-CGATGTGTAGGTTTCCG-3′
G. vaginalis Zariffard MR [28] F-GV1: 5′-TTACTGGTGTATCACTGTAAGG-3′ 16 S r RNA 15 min 95 °C, (45 sec 95 °C, 45 sec 55 °C, 45 sec 72 °C) x50 260 nM
R-GV3: 5′-CCGTCACAGGCTGAACAGT-3′
A. vaginae De Backer E [31] ATOVAGRT3Fw: 5′-GGTGAAGCAGTGGAAACACT-3′
ATOVAGRT3Rev: 5′-ATTCGCTTCTGCTCGCGCA-3′
16 S r RNA 15 min 95 °C, (20 sec 95 °C, 45 sec 60 °C, 45 sec 72 °C) x45 300 nM