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Figure 4 | BMC Microbiology

Figure 4

From: LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situhybridization

Figure 4

FISH staining of Arsenophonus 16 S rRNA in whole mount of whitefly Bemisia tabaci . (A.b) DNA probe stains Arsenophonus in the bacteriocytes; (B.b) at the same concentration (1.0 pmoles) LNA probe shows higher signal and a low background while staining for Arsenophonus. Arrows indicate the bacteriocytes. White arrowhead indicates the non-specific background in DNA samples. The images have been taken at best formamide concentration for Arsenophonus DNA (30%) and LNA (70%) probes separately. Both DNA and LNA panels also show merged and DIC images (as a and c respectively).

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BMC Microbiology

ISSN: 1471-2180

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