Signal to noise ratio of LNA and DNA probes while detecting the more abundant endosymbiont ( Portiera ). This graph depicts the signal to noise ratio, per 100 μm square area and plotted against increasing formamide concentration. No background correction was performed here. The value was calculated by dividing signal with the background of the same image and thus it gives a good idea about the binding efficiency of the probe. Here, LNA probe has a high signal to noise ratio at 60% formamide concentration followed by 30% formamide concentration, when compared to DNA probe. The signal of LNA probe is always high than the DNA probe at all formamide concentrations. Portiera was detected at 9 different formamide concentrations (0%-80%), both by DNA as well as the LNA probes. Fluorescence intensities were quantified by NIS elements (V 3.21.02) image analysis software (Nikon).