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Figure 2 | BMC Microbiology

Figure 2

From: LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situhybridization

Figure 2

Comparison between LNA and DNA probes while detecting the more abundant endosymbiont ( Portiera ). This graph depicts signal intensity profiles of LNA and DNA probes as a function of formamide concentration after background subtraction. At the same concentration, the intensity profile of LNA probe is significantly higher than the DNA probe while detecting Portiera, an endosymbiont of high abundance. All the images were acquired at fixed camera and microscope settings for DNA and LNA with Nikon A1 confocal microscope. Fluorescence intensities were quantified by NIS elements (V 3.21.02) image analysis software (Nikon).

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BMC Microbiology

ISSN: 1471-2180

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