FISH staining of Portiera 16 S rRNA in whole mount of whitefly Bemisia tabaci. FAM labeled oligonucleotide DNA probe and modified LNA probes were used to detect Portiera in B. tabaci. (A.b) DNA probe stains for Portiera in the bacteriocytes (B.b) at the same concentration (0.6 pmoles) LNA probe shows higher signal and lower background while staining for Portiera. Arrows indicate the bacteriocytes. The images have been taken at best formamide concentration for Portiera DNA (40%) and LNA (60%) probes separately. Both DNA and LNA panels also show merged and DIC images (as a and c respectively). All the images were acquired at fixed camera and microscope settings with Nikon A1 confocal microscope.