Analysis of apoptotic markers in Wt andgup1∆ chronologically aged cells. (A) Graphic representation of the percentage of cells displaying positive PI staining. (B) Phosphatidylserine externalization assessed by cytometric analysis of Annexin V labelling. Graphic representation of the percentage of cells displaying Ann V (+)/PI (−) (black bars), Ann V(+)/PI (+) (grey bars) and Ann V(−)/PI (+) (white bars). (C) Representative photos of DiOC6 staining exponential phase and aged cells. (D) Representative photos of DAPI staining of exponential phase and aged cells. For flow cytometry and fluorescence microscopy assays a minimum of 35,000 and 300 cells were counted, respectively. Data represent mean ± SD of 3 independent experiments.