Deletion of GUP1 decreases chronological lifespan. Wt (■) and gup1∆ mutant (∆).cells were inoculated in YNB medium and survival monitored by c.f.u. for 30 days (100% represents the 1,000 plated cells counting using a Neubauer chamber). The growth curve in YNB for both strains is presented in the insert. Data represent mean ± SD of at least 3 independent experiments.