Table 2 PCR primers used in this study
Primer | Sequence (5'-3') | Description |
|---|---|---|
1127 | GTTGAAGCAATATTGAAGAAAGC | sssF screen forward |
1128 | TTCTTCATTTAGTTTACCCATATCAAC | sssF screen reverse |
839 | GCTAGGATCCTCCATCTAATTCAAATGACAACG | sssF cloning forward. Contains BamHI site (underlined) |
840 | ACTAGGATCCGCTCCATTCAAAGTTCCACTTAC | sssF cloning reverse. Contains BamHI site (underlined) |
873 | GCTCACTCGAGTTCGACACCATCAGTAGAAGC | sssF fragment PCR for cloning into pBAD/HisB, for antibody production, forward. Contains XhoI site (underlined) |
874 | GCTCGGAATTCAAGCGCTTTAGCTTTAGCATC | sssF fragment PCR for cloning into pBAD/HisB, for antibody production, reverse. Contains EcoRI site (underlined) |
1001 | AAAAAAGCTTATAATTATCCTTAAGTCACTACTATGTGCGCCCAGATAGGGTG | sssF TargeTron IBS |
1002 | CAGATTGTACAAATGTGGTGATAACAGATAAGTCTACTATCTTAACTTACCTTTCTTTGT | sssF TargeTron EBS1d |
1003 | TGAACGCAAGTTTCTAATTTCGATTTGACTTCGATAGAGGAAAGTGTCT | sssF TargeTron EBS2 |
2065 | AAAAAAGCTTATAATTATCCTTATCGTACGGCAAGGTGCGCCCAGATAGGGTG | sasF TargeTron IBS |
2066 | CAGATTGTACAAATGTGGTGATAACAGATAAGTCGGCAAGATTAACTTACCTTTCTTTGT | sasF TargeTron EBS1d |
2067 | TGAACGCAAGTTTCTAATTTCGGTTTACGATCGATAGAGGAAAGTGTCT | sasF TargeTron EBS2 |
2084 | CAGTAAGCTTTGTTAGCGACATGGACAATATG | sasF cloning forward. Contains HindIII site (underlined) |
2085 | CCGTAAGCTTTTGCATATACTTCACAATAAATTAAGG | sasF cloning reverse. Contains HindIII site (underlined) |
1011 | TTCTTTAGGTGATGAACATATCAGG | Sequencing primer to check for correct 350 bp retargeted intron fragments for TargeTron |
EBSU | CGAAATTAGAAACTTGCGTTCAGTAAAC | TargeTron EBS universal |