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Table 3 Expression of β-glucuronidase (GUS) fusions

From: A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules

ORF strain % of nodules with GUS expression Strength of nodule GUS expression Staining time Pattern of nodule GUS expression Free-living GUS expression
N/A S. meliloti 1021 wild type (negative control) 0/39 = 0% variable none
SMc00911 SMc00911.original 18/20 = 90% ++++ 1.5–3.75 hr whole nodule +
  SMc00911.Xsd1 18/18 = 100% ++++ 1.5–3.75 hr whole nodule n.d.
  SMc00911.original2 n.d. n.d. N/A N/A +
SMb20360 SMb20360.original 8/13 = 62% ++ 3–5 hr invasion zone-fixation zone
  SMb20360.Xsd1 13/16 = 81% ++ 3–5 hr invasion zone-fixation zone
SMc00135 B104.3A 6/8 = 75% + 2–3 hr invasion zone-interzone +
  B104.4B 8/8 = 100% + 2–3 hr invasion zone-interzone ++
  B104.2 C 6/8 = 75% ++ 2–3 hr invasion zone-interzone ++
SMc01562 A104U.original 7/8 = 88% + 4–6 hr interzone
  A104U.Xsd1 3/7 = 43% +/− 4–6 hr interzone-fixation zone n.d.
  A104U.Xsd6 8/8 = 100% + 4–6 hr interzone-fixation zone n.d.
  A104U.Xsd25 3/8 = 38% +/− 4–6 hr interzone-fixation zone n.d.
  A104U.Xs100 4/9 = 44% + 4–6 hr fixation zone n.d.
SMc01266 SMc01266.original 13/18 = 72% + 3 hr invasion zone-fixation zone +/−
  SMc01266.Xsd1 13/18 = 72% ++ 3 hr invasion zone
SMc03964 SMc03964.original 8/15 = 53% ++ 3–5 hr interzone +/−
  SMc03964.Xsd6 9/19 = 47% ++ 3–5 hr interzone-fixation zone
SMc01424-22 D104.2A 0/8 = 0% 4–6 hr N/A +/−
  D104.3B 7/8 = 88% ++ 4–6 hr invasion zone-interzone +/−
  D104.1 C 6/8 = 75% + 4–6 hr invasion zone-fixation zone +/−
SMa0044 SMa0044.104.1A 4/8 = 50% +/− 6–7 hr invasion zone-interzone +++
  SMa0044.104.1B 4/8 = 50% +/− 6–7 hr interzone +++
  SMa0044.104.4 C 4/8% 50% +/− 6–7 hr interzone +++
SMb20431 SMb20431.original 10/16 = 63% + 5–12 hr invasion zone-fixation zone
  SMb20431.Xsd1 11/15 = 73% + 5–12 hr interzone
SMc01986 C104.1A.Xsd1 0/6 = 0% 24 hr N/A n.d.
  C104.1A.original n.d. n.d. 24 hr n.d. +/−
  C104.2B.Xsd100 2/18 = 11% +/− 24 hr fixation zone n.d.
SMa1334 SMa1334.original 0/11 = 0% 5–24 hr N/A
  SMa1334.Xsd1 0/13 = 0% 5–24 hr N/A