Table 3 Expression of β-glucuronidase (GUS) fusions
ORF | strain | % of nodules with GUS expression | Strength of nodule GUS expression | Staining time | Pattern of nodule GUS expression | Free-living GUS expression |
|---|---|---|---|---|---|---|
N/A | S. meliloti 1021 wild type (negative control) | 0/39 = 0% | − | variable | none | − |
SMc00911 | SMc00911.original | 18/20 = 90% | ++++ | 1.5–3.75 hr | whole nodule | + |
SMc00911.Xsd1 | 18/18 = 100% | ++++ | 1.5–3.75 hr | whole nodule | n.d. | |
SMc00911.original2 | n.d. | n.d. | N/A | N/A | + | |
SMb20360 | SMb20360.original | 8/13 = 62% | ++ | 3–5 hr | invasion zone-fixation zone | − |
SMb20360.Xsd1 | 13/16 = 81% | ++ | 3–5 hr | invasion zone-fixation zone | − | |
SMc00135 | B104.3A | 6/8 = 75% | + | 2–3 hr | invasion zone-interzone | + |
B104.4B | 8/8 = 100% | + | 2–3 hr | invasion zone-interzone | ++ | |
B104.2 C | 6/8 = 75% | ++ | 2–3 hr | invasion zone-interzone | ++ | |
SMc01562 | A104U.original | 7/8 = 88% | + | 4–6 hr | interzone | − |
A104U.Xsd1 | 3/7 = 43% | +/− | 4–6 hr | interzone-fixation zone | n.d. | |
A104U.Xsd6 | 8/8 = 100% | + | 4–6 hr | interzone-fixation zone | n.d. | |
A104U.Xsd25 | 3/8 = 38% | +/− | 4–6 hr | interzone-fixation zone | n.d. | |
A104U.Xs100 | 4/9 = 44% | + | 4–6 hr | fixation zone | n.d. | |
SMc01266 | SMc01266.original | 13/18 = 72% | + | 3 hr | invasion zone-fixation zone | +/− |
SMc01266.Xsd1 | 13/18 = 72% | ++ | 3 hr | invasion zone | − | |
SMc03964 | SMc03964.original | 8/15 = 53% | ++ | 3–5 hr | interzone | +/− |
SMc03964.Xsd6 | 9/19 = 47% | ++ | 3–5 hr | interzone-fixation zone | − | |
SMc01424-22 | D104.2A | 0/8 = 0% | − | 4–6 hr | N/A | +/− |
D104.3B | 7/8 = 88% | ++ | 4–6 hr | invasion zone-interzone | +/− | |
D104.1 C | 6/8 = 75% | + | 4–6 hr | invasion zone-fixation zone | +/− | |
SMa0044 | SMa0044.104.1A | 4/8 = 50% | +/− | 6–7 hr | invasion zone-interzone | +++ |
SMa0044.104.1B | 4/8 = 50% | +/− | 6–7 hr | interzone | +++ | |
SMa0044.104.4 C | 4/8% 50% | +/− | 6–7 hr | interzone | +++ | |
SMb20431 | SMb20431.original | 10/16 = 63% | + | 5–12 hr | invasion zone-fixation zone | − |
SMb20431.Xsd1 | 11/15 = 73% | + | 5–12 hr | interzone | − | |
SMc01986 | C104.1A.Xsd1 | 0/6 = 0% | − | 24 hr | N/A | n.d. |
C104.1A.original | n.d. | n.d. | 24 hr | n.d. | +/− | |
C104.2B.Xsd100 | 2/18 = 11% | +/− | 24 hr | fixation zone | n.d. | |
SMa1334 | SMa1334.original | 0/11 = 0% | − | 5–24 hr | N/A | − |
SMa1334.Xsd1 | 0/13 = 0% | − | 5–24 hr | N/A | − |