Table 2 S. meliloti 1021-derived mutant strains
ORF | Predicted function | Length (amino acids) | Type of mutation | Strain name |
|---|---|---|---|---|
SMc01562 | hypothetical protein | 96 | deletion | ΔSMc01562.6 |
ΔSMc01562.25 | ||||
ΔSMc01562.100 | ||||
SMc01562 | hypothetical protein | 96 | non-disrupting insertion of pJH104 GUS marker | A104U.original |
A104U.Xsd1 | ||||
A104U.Xsd6 | ||||
A104U.Xsd25 | ||||
A104U.Xs100 | ||||
SMc01986 | hypothetical protein | 119 | deletion | ΔSMc01986.1 |
ΔSMc01986.6 | ||||
ΔSMc01986.25 | ||||
ΔSMc01986.100 | ||||
SMc01986 | hypothetical protein | 119 | non-disrupting insertion of pJH104 GUS marker | C104.1A.Xsd1 |
C104.1A.original | ||||
C104.2B.Xsd100 | ||||
SMc00135 | hypothetical protein | 243 | deletion | ΔSMc00135.B1 |
ΔSMc00135.B17 | ||||
SMc00135 | hypothetical protein | 243 | non-disrupting insertion of pJH104 GUS marker | B104.3A |
B104.4B | ||||
B104.2 C | ||||
SMc01422 | hypothetical protein (probable operon with SMc01423,SMc01424) | 128 | deletion (SMc01422, SMc01423, SMc01424 all deleted in this strain) | ΔSMc01422-24.D21 ΔSMc01422-24.D29 |
SMc01423 | probable nitrile hydratase subunit β | 219 | deletion | same as above |
SMc01424 | probable nitrile hydratase subunit α | 213 | deletion | same as above |
SMc01424-01422 | hypothetical protein (probable operon with SMc01423,SMc01422) | 213 | non-disrupting insertion of pJH104 GUS marker | D104.2A |
D104.3B | ||||
D104.1 C | ||||
SMa0044 | hypothetical protein | 89 | deletion | ΔSMa0044.c1 |
ΔSMa0044.c6 | ||||
ΔSMa0044.c10 | ||||
SMa0044 | non-disrupting insertion of pJH104 GUS marker | 89 | SMa0044.104.1A | |
SMa0044.104.1B | ||||
SMa0044.104.4 C | ||||
SMb20431 | hypoth. arylmalonate decarboxylase | 261 | ORF-disrupting insertion of pJH104 GUS marker | SMb20431.original |
SMb20431.Xsd1 | ||||
SMb20360 | hypothetical protein | 243 | ORF-disrupting insertion of pJH104 GUS marker | SMb20360.original |
SMb20360.Xsd1 | ||||
SMc03964 | hypothetical protein | 300 | ORF-disrupting insertion of pJH104 GUS marker | SMc03964.original |
SMc03964.Xsd6 | ||||
SMc00911 | hypothetical protein | 275 | ORF-disrupting insertion of pJH104 GUS marker | SMc00911.original |
SMc00911.Xsd1 | ||||
SMc00911.original2 | ||||
SMa1334 | hypothetical protein | 398 | ORF-disrupting insertion of pJH104 GUS marker (may have a polar effect on 3′ genes Sma1332,-1331,-1329) | SMa1334.original |
SMa1334.Xsd1 | ||||
SMc01266 | hypothetical protein | 438 | ORF-disrupting insertion of pJH104 GUS marker (may have a polar effect on 3′ gene Smc01265) | SMc01266.original |
SMc01266.Xsd1 | ||||
greA | transcription elongation factor | 158 | ORF-disrupting insertion of pJH104 GUS marker | greA.12.4.1a |
expA1 (wgaA) | EPSII biosynthesis enzyme | 490 | ORF-disrupting insertion of Tn5-Nm in expA—symbiotically proficient, competitor assay strain | expA125::Tn5.Xsd1 |