Table 1 Strains and Plasmids

ATCC15692

ΔmexB derivative of PAO1

ΔlasI ΔrhlI derivative of PAO1

ΔlasI ΔrhlI ΔmexB derivative of PAO1

gfp fused to the lasB promoter and integrated at the attB site of the KG7004 chromosome

gfp fused to the lasB promoter and integrated at the attB site of the KG7050 chromosome

F^-, Φ80d lacZ Δ M15, Δ(lacZYA- argF')U169, deoR, recA 1, endA 1, hsdR17(r_k^- m_k⁺), phoA, supE 44, λ^-, thi-1, gyrA 96, relA 1

RE42-Tc: Mu-Km:: Tn7 pro res mod4

Ap^r; high-copy-number cloning vector

Ap^r Tc^r; high-copy-number cloning vector

super-polylinker phagemid

Gm^r; E. coli-P. aeruginosa shuttle expression vector

Cb^r; pBend2 derivative carrying multiple-cloning site and Not I site

Cm^r; pMOB3 derivative carrying Ω-Cm instead of Cm

Cb^r Cm^r; pKT5059 carrying 2911-bp fragment with 3′ flanking region of rhlI including 91-bp of rhlI and 2110-bp fragment with 5′ flanking region of rhlI Mob cassette from pMT5071 at Not I

Cb^r Cm^r; pMT5059 carrying 1.0-kb PCR fragments with 3′ and 5′ flanking regions of lasI and Mob cassette from pMT5071 at Not I

Cb^r Cm^r; pMT5059 carrying 1.0-kb PCR fragments with 3′ and 5′ flanking regions of mexB and Mob cassette from pMT5071 at Not I

Gm^r; subcloning of full length mexB in Xba I and Hin dIII site of pTO003

Phe136Ala mutation of mexB on pKTA113

Asp681Ala mutation of mexB on pKTA113

Ap^r; Enhanced GFP cassette plasmid

Tc^r; self-profcient integration vector with tet, V-FRT-attP-MCS, ori, int, and oriT

Tc^r; subcloning of gfp from pGreen

Tc^r; subcloning of a 598-bp lasB promoter region between Hin dIII and Kpn I sites of pSG

Ap^r; source of Flp recombinase

Tc^r; pVS1-p15A shuttle vector

Tc^r; subcloning of a 1781-bp lasB promoter::gfp region from pSQG003 in Bgl II site of pME6012