Coverage evaluation of universal bacterial primers using the metagenomic datasets

BMC Microbiology

Table 3 Detailed information for the 8 primers evaluated

Primer name	Degenerate type	Sequence of primer	Position in Escherichia coli	Reference (s)
27 F (8 F)	11Y12M	5′- AGA GTT TGA TYM TGG CTC AG-3′	8-27	[46]
338 F		5′-ACT CCT ACG GGA GGC AGC-3′	338-355	[47]
338R		5′-GCT GCC TCC CGT AGG AGT-3′	355-338	[48]
519 F	5 M	5′-CAG CM G CCG CGG TAA TAC-3′	519-536	[49]
519R (536R)	14 K	5′-GTA TTA CCG CGG CK G CTG-3′	536-519	[50]
907R (926R)	11 M	5′-CCG TCA ATT CM T TTG AGT TT-3′	926-907	[51]
1390R (1406R)	14R	5′-ACG GGC GGT GTG TR C AA-3′	1390-1406	[1, 52]
1492R	11Y	5′-TAC CTT GTT AY G ACT T-3′	1492-1507	[53, 54]

Alternative names for the primers are annotated in parentheses. In the “Degenerate type” column, the number and the capital letter denote the position and the content of the degenerate nucleotides. For example, primer 27 F is also known as 8 F, and “11Y12M” means that the 11th base is the degenerate nucleotide Y and the 12th base is M (Y = C or T, M = A or C, K = T or G and R = A or G).

ISSN: 1471-2180