Skip to main content

Table 1 Bacterial strains used in this study

From: Hypochlorous acid and hydrogen peroxide-induced negative regulation of Salmonella enterica serovar Typhimurium ompW by the response regulator ArcA

Strain Relevant characteristic(s) Source
S. Typhimurium   
14028s wild type strain G. Mora
14028s/pompW-lacZ 14028s transformed with a derivative of plasmid pLacZ-Basic carrying the ompW promoter (nt −600 to +1) This work
14028s/pompW/ABS1-lacZ 14028s transformed with a derivative of plasmid pLacZ-Basic carrying the ompW promoter (nt −600 to +1) with substitution GTTAA to TCCGG into position −70 to −66 This work
ΔompW ompW::kan C. Saavedra
ΔompW/pBAD-ompW ΔompW strain complemented with pBAD vector carrying the S. Typhimurium ompW gene C. Saavedra
ΔarcA arcA::cam [12]
ΔarcA/ pBAD-arcA ΔarcA strain complemented with pBAD vector carrying the S. Typhimurium arcA gene [12]
ΔarcB arcB::cam This work
ΔarcB/ pBAD-arcB ΔarcB strain complemented with pBAD vector carrying the S. Typhimurium arcB gene This work
E. coli
Top10
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
Top10 pBAD-ompW Top10 transformed with the pBAD vector carrying the S. Typhimurium ompW gene C. Saavedra
Top10 pBAD-ompA Top10 transformed with the pBAD vector carrying the S. Typhimurium ompA gene C. Saavedra
Top10 pBAD-arcB Top10 transformed with the pBAD vector carrying the S. Typhimurium arcB gene This work
BL21 pET-TOPOArcA BL21(DE3) transformed with the pET-TOPO101ArcA vector carrying the S. Typhimurium arcA gene [12]