Table 1 Bacterial strains used in this study
Strain | Relevant characteristic(s) | Source |
|---|---|---|
S. Typhimurium | ||
14028s | wild type strain | G. Mora |
14028s/pompW-lacZ | 14028s transformed with a derivative of plasmid pLacZ-Basic carrying the ompW promoter (nt −600 to +1) | This work |
14028s/pompW/ABS1-lacZ | 14028s transformed with a derivative of plasmid pLacZ-Basic carrying the ompW promoter (nt −600 to +1) with substitution GTTAA to TCCGG into position −70 to −66 | This work |
ΔompW | ompW::kan | C. Saavedra |
ΔompW/pBAD-ompW | ΔompW strain complemented with pBAD vector carrying the S. Typhimurium ompW gene | C. Saavedra |
ΔarcA | arcA::cam | [12] |
ΔarcA/ pBAD-arcA | ΔarcA strain complemented with pBAD vector carrying the S. Typhimurium arcA gene | [12] |
ΔarcB | arcB::cam | This work |
ΔarcB/ pBAD-arcB | ΔarcB strain complemented with pBAD vector carrying the S. Typhimurium arcB gene | This work |
E. coli Top10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
Top10 pBAD-ompW | Top10 transformed with the pBAD vector carrying the S. Typhimurium ompW gene | C. Saavedra |
Top10 pBAD-ompA | Top10 transformed with the pBAD vector carrying the S. Typhimurium ompA gene | C. Saavedra |
Top10 pBAD-arcB | Top10 transformed with the pBAD vector carrying the S. Typhimurium arcB gene | This work |
BL21 pET-TOPOArcA | BL21(DE3) transformed with the pET-TOPO101ArcA vector carrying the S. Typhimurium arcA gene | [12] |