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Figure 1 | BMC Microbiology

Figure 1

From: Hypochlorous acid and hydrogen peroxide-induced negative regulation of Salmonella enterica serovar Typhimurium ompW by the response regulator ArcA

Figure 1

OmpW facilitates H 2 O 2 and HOCl diffusion through the outer membrane and reconstituted proteoliposomes. A and C. H2O2 and HOCl levels were measured indirectly by specific fluorescence assays in the wild type (14028s), mutant (∆ompW) and genetically complemented strains (∆ompW/pBAD-ompW + arabinose). Exponentially growing cells were exposed to H2O2(A) or NaOCl (C) for 5 min and fluorescence was determined in the extracellular (extra) and intracellular fractions. B and D. Free liposomes (L), proteoliposomes reconstituted with S. Typhimurium OmpW (PL OmpW) or OmpA (PL OmpA) proteins were incubated with H2O2(B) or NaOCl (D) for 5 min and fluorescence was determined in the extraliposomal (extra) and intraliposomal fractions. AU indicates arbitrary units. Values represent the average of four independent experiments ± SD.

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BMC Microbiology

ISSN: 1471-2180

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