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Table 2 Primers used for construction

From: Construction of Bordetella pertussis strains with enhanced production of genetically-inactivated Pertussis Toxin and Pertactin by unmarked allelic exchange

Name

Sequence

5'F-PT-SalI

GCGGTCGACGGCGCGCAATGCGGCGCGGAC

5'R-PT-MCS

GGGGGCGGCCGCGAGATCTCTCTAGACGGTACCATCGCGCGACTTTGCGCCGAAGGA

3'F-PT-XbaI

CGTTCTAGACCTGGCCCAGCCCCGCCCAAC

3'R-PT-BglII

GGCAGATCTGCAGTTCGAGCAGATCGCCGG

CmF-KpnI

CGCGGTACCTGATGTCCGGCGGTGCTTTTG

CmR-XbaI

AATCTAGATATCGTCAATTATTACCTCCAC

S1F-PT-KpnI

GATGGTACCGGTCACCGTCCGGACCGTGCT

S1R-PT-XbaI

CAGGTCTAGAACGAATACGCGATGCTTTCG

R-R9K

GGGCGGGAGTCATACTTGTATACGGTGGCGG

F-R9K

CCGCCACCGTATACAAGTATGACTCCCGCCC

F-E129G

CCACCTACCAGAGCGGGTATCTGGCACACCGG

R-E129G

CCGGTGTGCCAGATACCCGCTCTGGTAGGTGG

5'F-PD-ApaI

GGAGGGCCCATGAAACTCGTCATCGCCATCATCAAGCCC

5'R-PD-MCS

TACGGTACCGGATCCCGCATCGCAACAACGGGGTCATCGCGACCC

3'F-PD-MCS

CGTTCTAGAACTAGTCCGCTACCAGGTGTAGCGATAGCCCAGGTG

3'R-PD-BglII

TGTAGATCTCGGCGAGATACTTGCGTTTCGGCGTTGTCG

PtxF-BamHI

TTGGGATCCCAGCGCAGCCCTCCAACGCGCCATCC

PtxR-MCS

TCTACTAGTAAGAATTCTCGCGGTATCCGTCAAGGAAAAACATGGAC

TerF-EcoRI

GCGGAATTCCGCCTGCCGCCTGCACGCAT

TerR-SpeI

TCCACTAGTCAAGGGCATCGGGCGCCGGC

5'F-PD2-SpeI

CGCACTAGTCTATTCCAGCGGCGGGTCGAAATGGC

5'R-PD2-MCS

CCCCAGGCGGCCGCTGTCTAGAGTGGATCCCAGGCCGATGCGTCCGCCGTGCAGGC

3'F-PD2-XbaI

ATCTCTAGAATGGGCACCTCGGCCACGCTGGCGCTG

3'R-PD2-NotI

AAGTATGCGGCCGCATGAGCGAAACCCTGTTGAAAGTATC

CmF-BamHI

CGCGGATCCTGATGTCCGGCGGTGCTTTTG

FHAproF-BamHI

TCTGGATCCCTGCGCTGGCACCCGCGGCGGGCCG

FHAR-MCS

GCCTCTAGATTCATATGATTCCGACCAGCGAAGTGAAGTAAT

PRNF-NdeI

CTGGTCGGCATATGAACATGTCTCTGTCACGCATTG

PRNF

ATGAACATGTCTCTGTCACGCATTGTCAAGG

PRNR-XbaI

GCCTCTAGAGCCTGGAGACTGGCACCGGCCAAGC

PrnProF-BamHI

CGGGGATCCGCACCCTGGCCTGCGGGGCGGGACC

PRNProR-NdeI

AGACATGTTCATATGGATGCCAGGTGGAGAGCAGA

5'F-int

CTAGCGTTCGCATACCAAATCCTTGC

5'RCM-int

CCGTAATATCCAGCTGAACGGTCTGG

3'FCM-int

TCTGTGATGGCTTCCATGTCGGCAG

3'R-int

AGCATGTTGCGGTGTTCCCGGAATG

5'FPD-int

ATGACGGAAAGCCGCATGGGCATTGGGTCC

3'RPD-int

TTCGTACGTGTTCAGGTGCCGATTGCCGG

5'FPD2-int

TGGGCTGGCTGTTCTGGCACGAAACG

3'RPD2-int

TTCATCGAATCGGCGCTGATCCTGGC

PRNF-int

AGGTGCAGCCATACATCAAGGCCAGC

  1. Sequences with bold letters are the recognition sites for various restriction enzymes used for each construction. Underlined sequences are the sequences of the new codons used for constructing mutant