Table 2 Bacteria and plasmids used in this study
Strain, plasmid or oligonucleotide | Relevant characteristics or sequence | Source, reference, or purpose |
|---|---|---|
E. coli strains | ||
DH5α | Culture collection | |
C. glutamicum strains | ||
ATCC 13032 | WTa | Culture collection |
Plasmids | ||
pEKEx3 | SpecR | [48] |
pEKEx3-bioYMN | pEKEx3 containing bioY, bioM and bioN | This work |
pGEM-T-easy | cloning vector, AmpR | Promega |
Oligonucleotides | ||
bio-operon_fw | GATCTAGAATTCAAATTTCAGCCCCCATCC | This work |
bio-operon_rev | GATCTAGGATCCCTGACCGCTGGTAACAAG | This work |
bioYMN-RBS_fw | AAGGAGATATAGATTTGTTGAACACTGTTCAGGTG | This work |
bioMN-RBS_fw | AGGAGATATAGATATGCCCGAGATCATTTTTGACAACAC | This work |
bioYMN_rev | TTAATCGCCGGCACCACGTGC | This work |
bioY_rev | CTATTTCTTACGGATGTCAGGGAATGC | This work |
bioM_rev | TCATTTCGCAGGTTCCGCC | This work |
bioY-int_fw | CACAGCGGGAGTGCCTATTGTTTT | This work |
bioY-int_rev | GAAGGACGAGACCCACGATG | This work |
bioM-int_rev | CAGCGATGATCACTTCTGGCTC | This work |
dnaE-fw | TGCCCTTCCGGCGATGTCCAA | [48] |
dnaE-rev | CTGGAACCATGTCGTCCTAGAG | [48] |