Characterization of the biotin uptake system encoded by the biotin-inducible bioYMN operon of Corynebacterium glutamicum

Table 1 Gene expression differences of C. glutamicum WT in response to biotin limitation, biotin excess or supplementation with dethiobiotin

Gene^a	Annotation^a	Relative mRNA level
		1 μg/l biotin	20000 μg/l biotin	dethiobiotin ^b
		200 μg/l biotin	200 μg/l biotin	biotin^b
cg0095	biotin synthase BioB	6.8	0.1	11.3
cg0096	hypothetical protein	5.5	0.2	3.6
cg0097	hypothetical protein	10.1	0.1	3.5
cg0126	hypothetical protein	0.5	n.d.	2.1
cg0486	ABC-type transporter. permease component	n.d.	0.5	n.d.
cg0634	ribosomal protein L15 RplO	0.4	n.d.	n.d.
cg1141	lactam utilization protein	n.d.	0.5	1.2
cg1142	transport system	2.1	0.4	1.2
cg1214	cysteine desulfhydrase/selenocysteine lyase NadS	1.9	0.5	1.3
cg1216	quinolate synthase A NadA	1.9	0.5	1.4
cg1218	ADP-ribose pyrophosphatase NdnR	2.1	0.4	2.0
cg1671	hypothetical protein	n.d.	2.0	0.3
cg2147	Biotin transport protein BioY	18.8	0.1	4.4
cg2148	Biotin transport protein BioM	4.9	0.2	2.6
cg2149	Biotin transport protein BioN	2.0	0.4	1.6
cg2320	predicted transcriptional regulator MarR family	2.0	0.5	1.6
cg2560	isocitrate lyase AceA	3.1	0.4	1.0
cg2747	metalloendopeptidases-like protein	n.d.	0.4	2.3
cg2883	SAM-dependent methyltransferase	2.2	0.2	n.d.
cg2884	putative dipeptide/tripeptide permease	8.9	0.1	5.6
cg2885	adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA	3.8	0.1	n.d.
cg3231	hypothetical protein	0.5	n.d.	n.d.
cg3289	thiol:disulfide interchange protein TlpA	0.4	n.d.	n.d.

^aGene numbers and annotations of the revised C. glutamicum genome published by NCBI as NC003450
^bRatio of the mRNA level in cells grown in CGXII with 200 μg/l dethiobiotin to that of cells grown with 200 μg/l biotin

ISSN: 1471-2180